Linked Life Data

19506091

Source:http://linkedlifedata.com/resource/pubmed/id/19506091

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2009-8-18
pubmed:abstractText
Mucin 1 (MUC1) is a glycoprotein in human endometrium and is abundant at the luminal epithelial surface in the receptive phase. It has a highly glycosylated ecto-domain that contains keratan sulfate chains, that disappears at the time of implantation. In addition, the glycoforms on MUC1 differ in fertile and infertile women. Therefore the aims of this study were investigations on glycosylation of MUC1 with the Thomsen-Friedenreich (TF) epitope on normal human endometrium throughout the menstrual cycle and binding of galectin-1 on the TF epitope in the endometrium and the expression of galectin-1 on the human oocyte. Human endometrial tissue was obtained from 54 premenopausal patients and was immunohistochemically analyzed with monoclonal antibodies against MUC1, TF epitope, galectin-1, and biotinylated galectin-1. In addition, human oocytes were analyzed for TF, galectin-1 expression, and galectin-1 binding. We identified a significant upregulation of MUC1 and TF epitope and, in addition, galectin-1 binding in glandular epithelium and epithelial apical surface tissue from proliferative to secretory phase. With double staining experiments, we identified a coexpression of TF and MUC1 in the early secretory phase and galectin-1 binding to TF during the same period of time. In addition we identified TF epitope and galectin-1 expression plus binding on the human oocyte and irregularly fertilized oocytes. Upregulation of TF epitope on the glandular epithelium and epithelial apical surface tissue in the secretory phase and binding of galectin-1 at the same time show the possibility of galectin-1-mediated trophectoderm binding to the endometrium within the window of implantation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-1554
pubmed:author
pubmed:issnType
Print
pubmed:volume
57
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
871-81
pubmed:dateRevised
2010-9-2
pubmed:meshHeading
pubmed-meshheading:19506091-Antigens, Tumor-Associated, Carbohydrate, pubmed-meshheading:19506091-Biotinylation, pubmed-meshheading:19506091-Cell Line, Tumor, pubmed-meshheading:19506091-Coculture Techniques, pubmed-meshheading:19506091-Endometrium, pubmed-meshheading:19506091-Epithelial Cells, pubmed-meshheading:19506091-Epitopes, pubmed-meshheading:19506091-Female, pubmed-meshheading:19506091-Fertilization, pubmed-meshheading:19506091-Galectin 1, pubmed-meshheading:19506091-Glycosylation, pubmed-meshheading:19506091-Humans, pubmed-meshheading:19506091-Immunohistochemistry, pubmed-meshheading:19506091-Menstrual Cycle, pubmed-meshheading:19506091-Mucin-1, pubmed-meshheading:19506091-Oocytes, pubmed-meshheading:19506091-Premenopause, pubmed-meshheading:19506091-Protein Binding, pubmed-meshheading:19506091-Zygote
pubmed:year
2009
pubmed:articleTitle
The human endometrium expresses the glycoprotein mucin-1 and shows positive correlation for Thomsen-Friedenreich epitope expression and galectin-1 binding.
pubmed:affiliation
1st Department of Obstetrics and Gynaecology, Ludwig-Maximilians-University Munich, Maistrasse 11, 80337 Munich, Germany. udo.jeschke@med.uni-muenchen.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't