| pubmed-article:19503620 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19503620 | lifeskim:mentions | umls-concept:C0162740 | lld:lifeskim |
| pubmed-article:19503620 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:19503620 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:19503620 | lifeskim:mentions | umls-concept:C0020980 | lld:lifeskim |
| pubmed-article:19503620 | lifeskim:mentions | umls-concept:C2698650 | lld:lifeskim |
| pubmed-article:19503620 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:19503620 | pubmed:dateCreated | 2009-6-8 | lld:pubmed |
| pubmed-article:19503620 | pubmed:abstractText | Digoxigenin is derived from a plant steroid hormone digoxin found in the plants Digitalis sp. Digoxigenin has been used successfully in labeling nucleic acids. In this experiment we optimized minimum probe requirement for a nonradioactive digoxigenin-based gene detection system in the model plant Arabidopsis thaliana. We showed that 1 microL of labeled probe was sufficient to hybridize onto 1-10 microg of target plasmid DNA. We also examined the sensitivity of labeled probe and showed that 2 microL of labeled probe was not able to hybridize with 1 microg of target DNA, although 2 microL of labeled probe was able to detect target DNA ranging from 2 to 10 microg. To test the efficacy of our optimization protocol, we used 1 microL of labeled plasmid DNA pU16893 harboring an Arabidopsis housekeeping gene elongation factor-1 and showed that the elongation factor-1 gene could be detected in Arabidopsis genome under various environmental conditions. This paper describes a nonradioactive in situ hybridization technique to detect nucleic acids in plants. | lld:pubmed |
| pubmed-article:19503620 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19503620 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19503620 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19503620 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19503620 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19503620 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19503620 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19503620 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19503620 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19503620 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19503620 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19503620 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19503620 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19503620 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19503620 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19503620 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19503620 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:19503620 | pubmed:issn | 1943-4731 | lld:pubmed |
| pubmed-article:19503620 | pubmed:author | pubmed-author:BasuChhandakC | lld:pubmed |
| pubmed-article:19503620 | pubmed:author | pubmed-author:HartShanion... | lld:pubmed |
| pubmed-article:19503620 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19503620 | pubmed:volume | 20 | lld:pubmed |
| pubmed-article:19503620 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19503620 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19503620 | pubmed:pagination | 96-100 | lld:pubmed |
| pubmed-article:19503620 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:19503620 | pubmed:meshHeading | pubmed-meshheading:19503620... | lld:pubmed |
| pubmed-article:19503620 | pubmed:meshHeading | pubmed-meshheading:19503620... | lld:pubmed |
| pubmed-article:19503620 | pubmed:meshHeading | pubmed-meshheading:19503620... | lld:pubmed |
| pubmed-article:19503620 | pubmed:meshHeading | pubmed-meshheading:19503620... | lld:pubmed |
| pubmed-article:19503620 | pubmed:meshHeading | pubmed-meshheading:19503620... | lld:pubmed |
| pubmed-article:19503620 | pubmed:meshHeading | pubmed-meshheading:19503620... | lld:pubmed |
| pubmed-article:19503620 | pubmed:meshHeading | pubmed-meshheading:19503620... | lld:pubmed |
| pubmed-article:19503620 | pubmed:meshHeading | pubmed-meshheading:19503620... | lld:pubmed |
| pubmed-article:19503620 | pubmed:meshHeading | pubmed-meshheading:19503620... | lld:pubmed |
| pubmed-article:19503620 | pubmed:meshHeading | pubmed-meshheading:19503620... | lld:pubmed |
| pubmed-article:19503620 | pubmed:meshHeading | pubmed-meshheading:19503620... | lld:pubmed |
| pubmed-article:19503620 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19503620 | pubmed:articleTitle | Optimization of a digoxigenin-based immunoassay system for gene detection in Arabidopsis thaliana. | lld:pubmed |
| pubmed-article:19503620 | pubmed:affiliation | School of Biological Sciences, University of Northern Colorado, Greeley, CO 80639, USA. | lld:pubmed |
| pubmed-article:19503620 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19503620 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
