Source:http://linkedlifedata.com/resource/pubmed/id/19503620
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2009-6-8
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pubmed:abstractText |
Digoxigenin is derived from a plant steroid hormone digoxin found in the plants Digitalis sp. Digoxigenin has been used successfully in labeling nucleic acids. In this experiment we optimized minimum probe requirement for a nonradioactive digoxigenin-based gene detection system in the model plant Arabidopsis thaliana. We showed that 1 microL of labeled probe was sufficient to hybridize onto 1-10 microg of target plasmid DNA. We also examined the sensitivity of labeled probe and showed that 2 microL of labeled probe was not able to hybridize with 1 microg of target DNA, although 2 microL of labeled probe was able to detect target DNA ranging from 2 to 10 microg. To test the efficacy of our optimization protocol, we used 1 microL of labeled plasmid DNA pU16893 harboring an Arabidopsis housekeeping gene elongation factor-1 and showed that the elongation factor-1 gene could be detected in Arabidopsis genome under various environmental conditions. This paper describes a nonradioactive in situ hybridization technique to detect nucleic acids in plants.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19503620-10457838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19503620-11157678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19503620-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19503620-16188960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19503620-16783595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19503620-17099842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19503620-8969835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19503620-9163723
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1943-4731
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
96-100
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:19503620-Arabidopsis,
pubmed-meshheading:19503620-DNA, Plant,
pubmed-meshheading:19503620-DNA Probes,
pubmed-meshheading:19503620-Digoxigenin,
pubmed-meshheading:19503620-Genes, Plant,
pubmed-meshheading:19503620-Genome, Plant,
pubmed-meshheading:19503620-Immunoassay,
pubmed-meshheading:19503620-In Situ Hybridization,
pubmed-meshheading:19503620-Peptide Elongation Factor 1,
pubmed-meshheading:19503620-Plasmids,
pubmed-meshheading:19503620-Sensitivity and Specificity
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pubmed:year |
2009
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pubmed:articleTitle |
Optimization of a digoxigenin-based immunoassay system for gene detection in Arabidopsis thaliana.
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pubmed:affiliation |
School of Biological Sciences, University of Northern Colorado, Greeley, CO 80639, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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