Source:http://linkedlifedata.com/resource/pubmed/id/19496789
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
10
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pubmed:dateCreated |
2009-9-8
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pubmed:abstractText |
To understand the roles of phosphoinositides [PtdIns] in phagocytosis of parasitic eukaryotes, we examined the interaction of phosphatidylinositol-3-phosphate [PtdIns(3)P] and putative PtdIns-P-binding proteins during phagocytosis in the enteric protozoan parasite Entamoeba histolytica. It was previously shown that phagocytosis in E. histolytica is indispensable for virulence and is inhibited by PtdIns 3-kinase inhibitors. We demonstrated by time-lapse live imaging that during the initiation of phagocytosis, the PtdIns(3)P biomarker GFP-Hrs-FYVE, was translocated to the phagocytic cup, phagosome, and to tunnel-like structures connecting the plasma membrane and phagosomes. E. histolytica possesses 12 FYVE domain-containing proteins (EhFP1-12), 11 of which also contain the RhoGEF/DH domain. Among them EhFP4 was shown to be recruited to the tunnel-like structures and to the proximal region of the phagosome. We further demonstrated that EhFP4 physically interacted with 4 of 10 predominant Rho/Rac small GTPases. Phosphoinositide binding assay showed that EhFP4 unexpectedly bound to PtdIns(4)P via the carboxyl-terminal domain and that the FYVE domain modulates the binding specificity of EhFP4 to PtdIns-P. Expression of the FYVE domain from EhFP4 inhibited phagocytosis while enhancement was observed when mammalian Hrs-FYVE domain was expressed. Altogether, we demonstrated that PtdIns(3)P, PtdIns(4)P and EhFP4 coordinately regulate phagocytosis and phagosome maturation in this parasitic eukaryote.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cytoskeletal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Guanine Nucleotide Exchange Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Protozoan Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/rho guanine nucleotide exchange...
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1462-5822
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1471-91
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pubmed:meshHeading |
pubmed-meshheading:19496789-Animals,
pubmed-meshheading:19496789-Cell Membrane,
pubmed-meshheading:19496789-Cytoskeletal Proteins,
pubmed-meshheading:19496789-Entamoeba histolytica,
pubmed-meshheading:19496789-Guanine Nucleotide Exchange Factors,
pubmed-meshheading:19496789-Microscopy, Video,
pubmed-meshheading:19496789-Phagocytosis,
pubmed-meshheading:19496789-Phagosomes,
pubmed-meshheading:19496789-Phosphatidylinositol Phosphates,
pubmed-meshheading:19496789-Protein Interaction Mapping,
pubmed-meshheading:19496789-Protein Structure, Tertiary,
pubmed-meshheading:19496789-Protozoan Proteins
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pubmed:year |
2009
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pubmed:articleTitle |
Phosphatidylinositol-phosphates mediate cytoskeletal reorganization during phagocytosis via a unique modular protein consisting of RhoGEF/DH and FYVE domains in the parasitic protozoon Entamoeba histolytica.
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pubmed:affiliation |
Department of Parasitology, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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