Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2009-6-3
pubmed:abstractText
Commercial HIV-1 genotypic resistance assays are very expensive, particularly for use in resource-constrained settings like India. Hence a cost effective in-house assay for drug resistance was validated against the standard ViroSeq HIV-1 Genotyping System 2.0 (Celera Diagnostics, CA, USA). A total of 50 samples were used for this evaluation (21 proficiency panels and 29 clinical isolates). Known resistance positions within HIV-1 protease (PR) region (1-99 codons) and HIV-1 reverse-transcriptase (RT) region (1-240 codons) were included. The results were analysed for each codon as follows: (i) concordant; (ii) partially concordant; (iii) indeterminate and (iv) discordant. A total of 2750 codons (55 codons per patient samplex50 samples) associated with drug resistance (1050 PR and 1700 RT) were analysed. For PR, 99% of the codon results were concordant and 1% were partially concordant. For RT, 99% of the codon results were concordant, 0.9% were partially concordant and 0.1% were discordant. No indeterminate results were observed and the results were reproducible. Overall, the in-house assay provided comparable results to those of US FDA approved ViroSeq, which costs about a half of the commercial assay ($ 100 vs. $ 230), making it suitable for resource-limited settings.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19490976, http://linkedlifedata.com/resource/pubmed/commentcorrection/19490976-10364600, http://linkedlifedata.com/resource/pubmed/commentcorrection/19490976-10392984, http://linkedlifedata.com/resource/pubmed/commentcorrection/19490976-10501116, http://linkedlifedata.com/resource/pubmed/commentcorrection/19490976-10894268, http://linkedlifedata.com/resource/pubmed/commentcorrection/19490976-11807305, http://linkedlifedata.com/resource/pubmed/commentcorrection/19490976-11907381, http://linkedlifedata.com/resource/pubmed/commentcorrection/19490976-11923366, http://linkedlifedata.com/resource/pubmed/commentcorrection/19490976-12167680, http://linkedlifedata.com/resource/pubmed/commentcorrection/19490976-12442164, http://linkedlifedata.com/resource/pubmed/commentcorrection/19490976-12445937, http://linkedlifedata.com/resource/pubmed/commentcorrection/19490976-12822128, http://linkedlifedata.com/resource/pubmed/commentcorrection/19490976-15186710, http://linkedlifedata.com/resource/pubmed/commentcorrection/19490976-16208003, http://linkedlifedata.com/resource/pubmed/commentcorrection/19490976-16231268, http://linkedlifedata.com/resource/pubmed/commentcorrection/19490976-16375980, http://linkedlifedata.com/resource/pubmed/commentcorrection/19490976-16377851, http://linkedlifedata.com/resource/pubmed/commentcorrection/19490976-1709563, http://linkedlifedata.com/resource/pubmed/commentcorrection/19490976-17197878, http://linkedlifedata.com/resource/pubmed/commentcorrection/19490976-17653265, http://linkedlifedata.com/resource/pubmed/commentcorrection/19490976-18370589, http://linkedlifedata.com/resource/pubmed/commentcorrection/19490976-18852475, http://linkedlifedata.com/resource/pubmed/commentcorrection/19490976-18953272, http://linkedlifedata.com/resource/pubmed/commentcorrection/19490976-9634429
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1879-0984
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
159
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
211-6
pubmed:dateRevised
2011-6-6
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Evaluation of two human immunodeficiency virus-1 genotyping systems: ViroSeq 2.0 and an in-house method.
pubmed:affiliation
YRG Centre for AIDS Research and Education, Chennai 600 113, India.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't, Evaluation Studies