Linked Life Data

19488025

Source:http://linkedlifedata.com/resource/pubmed/id/19488025

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
27
pubmed:dateCreated
2009-6-2
pubmed:abstractText
Aging is a degenerative process characterized by a progressive deterioration of cellular components and organelles resulting in mortality. The nematode Caenorhabditis elegans has emerged as a principal model used to study the biology of aging. Because virtually every biological subsystem undergoes functional decline with increasing age, life span is the primary endpoint of interest when considering total rate of aging. In nematodes, life span is typically defined as the number of days an animal remains responsive to external stimuli. Nematodes can be propagated either in liquid media or on solid media in plates, and techniques have been developed for measuring life span under both conditions. Here we present a generalized protocol for measuring life span of nematodes maintained on solid nematode growth media and fed a diet of UV-killed bacteria. These procedures can easily be adapted to assay life span under various common conditions, including a diet consisting of live bacteria, dietary restriction, and RNA interference.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19488025-12136014, http://linkedlifedata.com/resource/pubmed/commentcorrection/19488025-12447374, http://linkedlifedata.com/resource/pubmed/commentcorrection/19488025-12471266, http://linkedlifedata.com/resource/pubmed/commentcorrection/19488025-15329389, http://linkedlifedata.com/resource/pubmed/commentcorrection/19488025-15998808, http://linkedlifedata.com/resource/pubmed/commentcorrection/19488025-16103914, http://linkedlifedata.com/resource/pubmed/commentcorrection/19488025-17248881, http://linkedlifedata.com/resource/pubmed/commentcorrection/19488025-17411345, http://linkedlifedata.com/resource/pubmed/commentcorrection/19488025-17521386, http://linkedlifedata.com/resource/pubmed/commentcorrection/19488025-18083317, http://linkedlifedata.com/resource/pubmed/commentcorrection/19488025-18331616, http://linkedlifedata.com/resource/pubmed/commentcorrection/19488025-18457595, http://linkedlifedata.com/resource/pubmed/commentcorrection/19488025-7126532, http://linkedlifedata.com/resource/pubmed/commentcorrection/19488025-926867
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1940-087X
pubmed:author
pubmed:issnType
Electronic
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Measuring Caenorhabditis elegans life span on solid media.
pubmed:affiliation
Department of Pathology, University of Washington, Seattle, WA, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural, Video-Audio Media