Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2009-6-2
pubmed:abstractText
High-risk human papillomavirus (HPV) infections are necessary but insufficient causes of cervical cancers. Other risk factors for cervical cancer (e.g., pregnancy, smoking, infections causing inflammation) can lead to high and sustained nitric oxide (NO) concentrations in the cervix, and high NO levels are related to carcinogenesis through DNA damage and mutation. However, the effects of NO exposure in HPV-infected cells have not been investigated. In this study, we used the NO donor DETA-NO to model NO exposure to cervical epithelium. In cell culture media, 24-hour exposure to 0.25 to 0.5 mmol/L DETA-NO yielded a pathologically relevant NO concentration. Exposure of cells maintaining episomal high-risk HPV genomes to NO increased HPV early transcript levels 2- to 4-fold but did not increase viral DNA replication. Accompanying increased E6 and E7 mRNA levels were significant decreases in p53 and pRb protein levels, lower apoptotic indices, increased DNA double-strand breaks, and higher mutation frequencies when compared with HPV-negative cells. We propose that NO is a molecular cofactor with HPV infection in cervical carcinogenesis, and that modifying local NO cervical concentrations may constitute a strategy whereby HPV-related cancer can be reduced.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1538-7445
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
69
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4878-84
pubmed:meshHeading
pubmed-meshheading:19487298-3T3 Cells, pubmed-meshheading:19487298-Animals, pubmed-meshheading:19487298-Cells, Cultured, pubmed-meshheading:19487298-Cervix Uteri, pubmed-meshheading:19487298-DNA Damage, pubmed-meshheading:19487298-Dose-Response Relationship, Drug, pubmed-meshheading:19487298-Epithelial Cells, pubmed-meshheading:19487298-Female, pubmed-meshheading:19487298-Gene Expression Regulation, Viral, pubmed-meshheading:19487298-Genes, Viral, pubmed-meshheading:19487298-Genome, Viral, pubmed-meshheading:19487298-Humans, pubmed-meshheading:19487298-Mice, pubmed-meshheading:19487298-Mutation, pubmed-meshheading:19487298-Nitric Oxide, pubmed-meshheading:19487298-Papillomavirus Infections, pubmed-meshheading:19487298-Retinoblastoma Protein, pubmed-meshheading:19487298-Transcription, Genetic, pubmed-meshheading:19487298-Tumor Suppressor Protein p53, pubmed-meshheading:19487298-Up-Regulation
pubmed:year
2009
pubmed:articleTitle
Nitric oxide induces early viral transcription coincident with increased DNA damage and mutation rates in human papillomavirus-infected cells.
pubmed:affiliation
Department of Molecular Genetics and Microbiology, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural