19481435

Source:http://linkedlifedata.com/resource/pubmed/id/19481435

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0009528, umls-concept:C0023693, umls-concept:C0037812, umls-concept:C0043157, umls-concept:C0086418, umls-concept:C0220938, umls-concept:C0229671, umls-concept:C0596035, umls-concept:C1511790
pubmed:issue	11
pubmed:dateCreated	2009-6-8
pubmed:abstractText	We present a label-free, real-time sensor based on white light reflectance spectroscopy for quantitating the complement activation product C3b and its metabolites as a biomarker in human serum. Our novel sensor allows real-time monitoring of biomolecular reactions (in this case, antigen-antibody reactions) taking place on a reflective surface within a flow cell. Detection was based on monitoring the increase in film thickness caused by its immunoreaction with a specific antibody; this reaction was seen as a shift in the wavelength at which constructive interference was observed. Quantitation of C3b was achieved by immobilizing a specific mouse monoclonal antibody onto the refractive surface and monitoring the rate of the signal changes occurring during the first 60s of the immunoreaction between the antibody and known concentrations of purified C3b or dilutions of complement-activated human serum. The lowest detectable concentration of purified C3b was 20 ng/mL, and complement activation products in human serum samples could be detected at dilutions as high as 6000-fold. The advantages of the method include its relatively low cost, short analysis time, and high assay sensitivity and reliability. Thus, this novel assay method can be used to monitor serum C3b produced as a result of complement activation in a variety of normal and pathologic conditions.
pubmed:grant	http://linkedlifedata.com/resource/pubmed/grant/AI068730, http://linkedlifedata.com/resource/pubmed/grant/AI071028, http://linkedlifedata.com/resource/pubmed/grant/AI30040, http://linkedlifedata.com/resource/pubmed/grant/P01 AI068730-02, http://linkedlifedata.com/resource/pubmed/grant/R01 AI030040-14, http://linkedlifedata.com/resource/pubmed/grant/R01 AI072106-02
pubmed:commentsCorrections	http://linkedlifedata.com/resource/pubmed/commentcorrection/19481435-11414361, http://linkedlifedata.com/resource/pubmed/commentcorrection/19481435-11939217, http://linkedlifedata.com/resource/pubmed/commentcorrection/19481435-12297420, http://linkedlifedata.com/resource/pubmed/commentcorrection/19481435-12918056, http://linkedlifedata.com/resource/pubmed/commentcorrection/19481435-1371073, http://linkedlifedata.com/resource/pubmed/commentcorrection/19481435-14675043, http://linkedlifedata.com/resource/pubmed/commentcorrection/19481435-15104126, http://linkedlifedata.com/resource/pubmed/commentcorrection/19481435-15159060, http://linkedlifedata.com/resource/pubmed/commentcorrection/19481435-15454921, http://linkedlifedata.com/resource/pubmed/commentcorrection/19481435-15488947, http://linkedlifedata.com/resource/pubmed/commentcorrection/19481435-15961157, http://linkedlifedata.com/resource/pubmed/commentcorrection/19481435-1688755, http://linkedlifedata.com/resource/pubmed/commentcorrection/19481435-17027250, http://linkedlifedata.com/resource/pubmed/commentcorrection/19481435-17172439, http://linkedlifedata.com/resource/pubmed/commentcorrection/19481435-17618110, http://linkedlifedata.com/resource/pubmed/commentcorrection/19481435-17640961, http://linkedlifedata.com/resource/pubmed/commentcorrection/19481435-17890075, http://linkedlifedata.com/resource/pubmed/commentcorrection/19481435-17892218, http://linkedlifedata.com/resource/pubmed/commentcorrection/19481435-17989689, http://linkedlifedata.com/resource/pubmed/commentcorrection/19481435-17999039, http://linkedlifedata.com/resource/pubmed/commentcorrection/19481435-2410510, http://linkedlifedata.com/resource/pubmed/commentcorrection/19481435-2457622, http://linkedlifedata.com/resource/pubmed/commentcorrection/19481435-266208, http://linkedlifedata.com/resource/pubmed/commentcorrection/19481435-2932466, http://linkedlifedata.com/resource/pubmed/commentcorrection/19481435-6783652, http://linkedlifedata.com/resource/pubmed/commentcorrection/19481435-9041627
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/9001289
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/Complement C3b
pubmed:status	MEDLINE
pubmed:month	Jul
pubmed:issn	1873-4235
pubmed:author	pubmed-author:KakabakosSotirios ESE, pubmed-author:LambrisJohn DJD, pubmed-author:MisiakosKonstantinosK, pubmed-author:PetrouPanagiota SPS, pubmed-author:RaptisIoannisI, pubmed-author:RicklinDanielD, pubmed-author:ZavaliMariaM
pubmed:issnType	Electronic
pubmed:day	15
pubmed:volume	24
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	3359-64
pubmed:dateRevised	2011-4-29
pubmed:meshHeading	pubmed-meshheading:19481435-Biosensing Techniques, pubmed-meshheading:19481435-Blood Chemical Analysis, pubmed-meshheading:19481435-Complement C3b, pubmed-meshheading:19481435-Computer Systems, pubmed-meshheading:19481435-Immunoassay, pubmed-meshheading:19481435-Light, pubmed-meshheading:19481435-Photometry, pubmed-meshheading:19481435-Sensitivity and Specificity, pubmed-meshheading:19481435-Spectrum Analysis, pubmed-meshheading:19481435-Staining and Labeling
pubmed:year	2009
pubmed:articleTitle	Real-time label-free detection of complement activation products in human serum by white light reflectance spectroscopy.
pubmed:affiliation	Institute of Radioisotopes and Radiodiagnostic Products, NCSR Demokritos, GR-15310 Aghia Paraskevi, Greece.
pubmed:publicationType	Journal Article, Evaluation Studies, Research Support, N.I.H., Extramural