| pubmed-article:19481283 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19481283 | lifeskim:mentions | umls-concept:C1265277 | lld:lifeskim |
| pubmed-article:19481283 | lifeskim:mentions | umls-concept:C0026131 | lld:lifeskim |
| pubmed-article:19481283 | lifeskim:mentions | umls-concept:C0030940 | lld:lifeskim |
| pubmed-article:19481283 | lifeskim:mentions | umls-concept:C0019409 | lld:lifeskim |
| pubmed-article:19481283 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:19481283 | pubmed:dateCreated | 2009-6-29 | lld:pubmed |
| pubmed-article:19481283 | pubmed:abstractText | Pseudomonas fragi, Pseudomonas lundensis and members of the Pseudomonas fluorescens group may spoil Ultra High Temperature (UHT) treated milk and dairy products, due to the production of heat-stable proteases in the cold chain of raw milk. Since the aprX gene codes for a heat-resistant protease in P. fluorescens, the presence of this gene has also been investigated in other members of the genus. For this purpose an aprX-screening PCR test has been developed. Twenty-nine representatives of important milk Pseudomonas species and thirty-five reference strains were screened. In 42 out of 55 investigated Pseudomonas strains, the aprX gene was detected, which proves the potential of the aprX-PCR test as a screening tool for potentially proteolytic Pseudomonas strains in milk samples. An extensive study of the obtained aprX-sequences on the DNA and the amino acid level, however, revealed a large heterogeneity within the investigated milk isolates. Although this heterogeneity sets limitations to a general detection method for all proteolytic Pseudomonas strains in milk, it offers a great potential for the development of a multiplex PCR screening test targeting individual aprX-genes. Furthermore, our data illustrated the potential use of the aprX gene as a taxonomic marker, which may help in resolving the current taxonomic deadlock in the P. fluorescens group. | lld:pubmed |
| pubmed-article:19481283 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19481283 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19481283 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19481283 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19481283 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19481283 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19481283 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19481283 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19481283 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:19481283 | pubmed:issn | 1879-3460 | lld:pubmed |
| pubmed-article:19481283 | pubmed:author | pubmed-author:DevreeseBartB | lld:pubmed |
| pubmed-article:19481283 | pubmed:author | pubmed-author:De VosPaulP | lld:pubmed |
| pubmed-article:19481283 | pubmed:author | pubmed-author:HeyndrickxMar... | lld:pubmed |
| pubmed-article:19481283 | pubmed:author | pubmed-author:MarchandSophi... | lld:pubmed |
| pubmed-article:19481283 | pubmed:author | pubmed-author:De BlockJanJ | lld:pubmed |
| pubmed-article:19481283 | pubmed:author | pubmed-author:DewettinckKoe... | lld:pubmed |
| pubmed-article:19481283 | pubmed:author | pubmed-author:De... | lld:pubmed |
| pubmed-article:19481283 | pubmed:author | pubmed-author:CoorevitsAnA | lld:pubmed |
| pubmed-article:19481283 | pubmed:author | pubmed-author:CoudijzerKatl... | lld:pubmed |
| pubmed-article:19481283 | pubmed:author | pubmed-author:VandriescheGo... | lld:pubmed |
| pubmed-article:19481283 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19481283 | pubmed:day | 31 | lld:pubmed |
| pubmed-article:19481283 | pubmed:volume | 133 | lld:pubmed |
| pubmed-article:19481283 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19481283 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19481283 | pubmed:pagination | 68-77 | lld:pubmed |
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| pubmed-article:19481283 | pubmed:meshHeading | pubmed-meshheading:19481283... | lld:pubmed |
| pubmed-article:19481283 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19481283 | pubmed:articleTitle | Heterogeneity of heat-resistant proteases from milk Pseudomonas species. | lld:pubmed |
| pubmed-article:19481283 | pubmed:affiliation | Institute for Agricultural and Fisheries Research, Melle, Belgium. sophie.marchand@ilvo.vlaanderen.be | lld:pubmed |
| pubmed-article:19481283 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19481283 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
