Source:http://linkedlifedata.com/resource/pubmed/id/19481283
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
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| pubmed:dateCreated |
2009-6-29
|
| pubmed:abstractText |
Pseudomonas fragi, Pseudomonas lundensis and members of the Pseudomonas fluorescens group may spoil Ultra High Temperature (UHT) treated milk and dairy products, due to the production of heat-stable proteases in the cold chain of raw milk. Since the aprX gene codes for a heat-resistant protease in P. fluorescens, the presence of this gene has also been investigated in other members of the genus. For this purpose an aprX-screening PCR test has been developed. Twenty-nine representatives of important milk Pseudomonas species and thirty-five reference strains were screened. In 42 out of 55 investigated Pseudomonas strains, the aprX gene was detected, which proves the potential of the aprX-PCR test as a screening tool for potentially proteolytic Pseudomonas strains in milk samples. An extensive study of the obtained aprX-sequences on the DNA and the amino acid level, however, revealed a large heterogeneity within the investigated milk isolates. Although this heterogeneity sets limitations to a general detection method for all proteolytic Pseudomonas strains in milk, it offers a great potential for the development of a multiplex PCR screening test targeting individual aprX-genes. Furthermore, our data illustrated the potential use of the aprX gene as a taxonomic marker, which may help in resolving the current taxonomic deadlock in the P. fluorescens group.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Jul
|
| pubmed:issn |
1879-3460
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| pubmed:author |
pubmed-author:CoorevitsAnA,
pubmed-author:CoudijzerKatleenK,
pubmed-author:De BlockJanJ,
pubmed-author:De JongheValerieV,
pubmed-author:De VosPaulP,
pubmed-author:DevreeseBartB,
pubmed-author:DewettinckKoenK,
pubmed-author:HeyndrickxMarcM,
pubmed-author:MarchandSophieS,
pubmed-author:VandriescheGonzalezG
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| pubmed:issnType |
Electronic
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| pubmed:day |
31
|
| pubmed:volume |
133
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
68-77
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| pubmed:meshHeading |
pubmed-meshheading:19481283-Adaptation, Physiological,
pubmed-meshheading:19481283-Amino Acid Sequence,
pubmed-meshheading:19481283-Animals,
pubmed-meshheading:19481283-Bacterial Proteins,
pubmed-meshheading:19481283-Base Sequence,
pubmed-meshheading:19481283-Cattle,
pubmed-meshheading:19481283-Cold Temperature,
pubmed-meshheading:19481283-DNA, Bacterial,
pubmed-meshheading:19481283-Food Microbiology,
pubmed-meshheading:19481283-Genes, Bacterial,
pubmed-meshheading:19481283-Genetic Variation,
pubmed-meshheading:19481283-Hot Temperature,
pubmed-meshheading:19481283-Humans,
pubmed-meshheading:19481283-Milk,
pubmed-meshheading:19481283-Phylogeny,
pubmed-meshheading:19481283-Polymerase Chain Reaction,
pubmed-meshheading:19481283-Pseudomonas,
pubmed-meshheading:19481283-Refrigeration,
pubmed-meshheading:19481283-Serine Endopeptidases
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| pubmed:year |
2009
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| pubmed:articleTitle |
Heterogeneity of heat-resistant proteases from milk Pseudomonas species.
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| pubmed:affiliation |
Institute for Agricultural and Fisheries Research, Melle, Belgium. sophie.marchand@ilvo.vlaanderen.be
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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