Linked Life Data

19480949

Source:http://linkedlifedata.com/resource/pubmed/id/19480949

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2009-6-1
pubmed:abstractText
The efficient use of microalgae to convert sun light energy into biomass is limited by losses during high light illumination of dense cell cultures in closed bioreactors. Uneven light distribution can be overcome by using cell cultures with smaller antenna sizes packed to high cell density cultures, thus allowing good light penetration into the inner sections of the reactor. We engineered a new small PSII antenna size Chlamydomonas reinhardtii strain with improved photon conversion efficiency and increased growth rates under high light conditions. We achieved this goal by transformation of a permanently active variant NAB1* of the LHC translation repressor NAB1 to reduce antenna size via translation repression. NAB1* expression was demonstrated in Stm6Glc4T7 (T7), leading to a reduction of LHC antenna size by 10-17%. T7 showed a approximately 50% increase of photosynthetic efficiency (PhiPSII) at saturating light intensity compared to the parental strain. T7 converted light to biomass with much higher efficiencies with a approximately 50% improved mid log growth phase. Moreover, T7 cultures reached higher densities when grown in large-scale bioreactors. Thus, the phenotype of strain T7 may have important implications for biotechnological applications in which photosynthetic microalgae are used for large-scale culturing as an alternative plant biomass source.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1873-4863
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
142
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
70-7
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Improvement of light to biomass conversion by de-regulation of light-harvesting protein translation in Chlamydomonas reinhardtii.
pubmed:affiliation
Department of Biology, Algae Biotech Group, University Bielefeld, 33501 Bielefeld, Germany.
pubmed:publicationType
Journal Article