Linked Life Data

19478046

Source:http://linkedlifedata.com/resource/pubmed/id/19478046

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2009-8-21
pubmed:abstractText
The megakaryocytic (MK) and erythroid lineages are tightly associated during differentiation and are generated from a bipotent megakaryocyte-erythroid progenitor (MEP). In the mouse, a primitive MEP has been demonstrated in the yolk sac. In human, it is not known whether the primitive MK and erythroid lineages are generated from a common progenitor or independently. Using hematopoietic differentiation of human embryonic stem cells on the OP9 cell line, we identified a primitive MEP in a subset of cells coexpressing glycophorin A (GPA) and CD41 from day 9 to day 12 of coculturing. This MEP differentiates into primitive erythroid (GPA(+)CD41(-)) and MK (GPA(-)CD41(+)) lineages. In contrast to erythropoietin (EPO)-dependent definitive hematopoiesis, KIT was not detected during erythroid differentiation. A molecular signature for the commitment and differentiation toward both the erythroid and MK lineages was detected by assessing expression of transcription factors, thrombopoietin receptor (MPL) and erythropoietin receptor (EPOR). We showed an inverse correlation between FLI1 and both KLF1 and EPOR during primitive erythroid and MK differentiation, similar to definitive hematopoiesis. This novel MEP differentiation system may allow an in-depth exploration of the molecular bases of erythroid and MK commitment and differentiation.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1528-0020
pubmed:author
pubmed:issnType
Electronic
pubmed:day
20
pubmed:volume
114
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1506-17
pubmed:meshHeading
pubmed-meshheading:19478046-Animals, pubmed-meshheading:19478046-Antigens, CD34, pubmed-meshheading:19478046-Antigens, CD43, pubmed-meshheading:19478046-Cell Differentiation, pubmed-meshheading:19478046-Cell Lineage, pubmed-meshheading:19478046-Cells, Cultured, pubmed-meshheading:19478046-Coculture Techniques, pubmed-meshheading:19478046-Embryonic Stem Cells, pubmed-meshheading:19478046-Erythroid Cells, pubmed-meshheading:19478046-Glycophorin, pubmed-meshheading:19478046-Hematopoiesis, pubmed-meshheading:19478046-Humans, pubmed-meshheading:19478046-Megakaryocyte-Erythroid Progenitor Cells, pubmed-meshheading:19478046-Megakaryocytes, pubmed-meshheading:19478046-Mice, pubmed-meshheading:19478046-Platelet Glycoprotein GPIb-IX Complex, pubmed-meshheading:19478046-Platelet Membrane Glycoprotein IIb
pubmed:year
2009
pubmed:articleTitle
A common bipotent progenitor generates the erythroid and megakaryocyte lineages in embryonic stem cell-derived primitive hematopoiesis.
pubmed:affiliation
Inserm U790, Institut Gustave Roussy, Villejuif, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't