Source:http://linkedlifedata.com/resource/pubmed/id/19470878
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2009-6-18
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| pubmed:abstractText |
Growth-differentiation factor 15 (GDF-15), a stress-responsive transforming growth factor-beta-related cytokine, is emerging as a new risk marker in patients with cardiovascular disease. We explored GDF-15 in preeclampsia and in diabetic pregnancies, because these conditions are associated with augmented risk for cardiovascular disease, both in mother and in offspring. Plasma from pregnant women (n=267; controls: n=59, preeclampsia: n=85, diabetes mellitus: n=112, and superimposed preeclampsia in diabetes mellitus: n=11), fetal plasma (n=72), and amniotic fluid (n=99) were analyzed by immunoassay for GDF-15. Placental GDF-15 mRNA and protein expression levels were analyzed by quantitative real-time PCR and immunoblots in 78 and 18 pregnancies, respectively. Conditioned media from preeclamptic (n=6) and control (n=6) villous placenta explants were analyzed by immunoassay for GDF-15. Median maternal GDF-15 concentration was elevated in those with diabetes mellitus, as compared with controls (91 549 versus 79 875 ng/L; P=0.02). Median GDF-15 concentration was higher in patients with preeclampsia than in controls in term maternal blood samples (127 061 versus 80 319 ng/L; P<0.001). In the fetal circulation and amniotic fluid, GDF-15 was elevated in preeclampsia and superimposed preeclampsia in diabetes mellitus, as compared with controls. GDF-15 placental mRNA expression was elevated in preeclampsia, as compared with controls (P=0.002). Placenta immunoblots confirmed a single GDF-15 protein band, and a time-dependent increase in GDF-15 protein was detected in the conditioned media. Our study is the first to show that GDF-15 is dysregulated, both in preeclampsia and in diabetic pregnancies. The mechanisms and diagnostic implications of these findings remain to be explored.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Jul
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| pubmed:issn |
1524-4563
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| pubmed:author |
pubmed-author:AntonLaurenL,
pubmed-author:BrosnihanK BridgetKB,
pubmed-author:DechendRalfR,
pubmed-author:HerseFlorianF,
pubmed-author:JohnsenGuro MGM,
pubmed-author:KempfTiborT,
pubmed-author:LuftFriedrich CFC,
pubmed-author:StaffAnne CathrineAC,
pubmed-author:SugulleMeryamM,
pubmed-author:Weedon-FekjaerM SusanneMS,
pubmed-author:WollertKai CKC
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| pubmed:issnType |
Electronic
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| pubmed:volume |
54
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
106-12
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| pubmed:meshHeading |
pubmed-meshheading:19470878-Adult,
pubmed-meshheading:19470878-Amniotic Fluid,
pubmed-meshheading:19470878-Culture Media, Conditioned,
pubmed-meshheading:19470878-Female,
pubmed-meshheading:19470878-Fetal Blood,
pubmed-meshheading:19470878-Growth Differentiation Factor 15,
pubmed-meshheading:19470878-Humans,
pubmed-meshheading:19470878-Immunoassay,
pubmed-meshheading:19470878-Immunoblotting,
pubmed-meshheading:19470878-Infant, Newborn,
pubmed-meshheading:19470878-Placenta,
pubmed-meshheading:19470878-Pre-Eclampsia,
pubmed-meshheading:19470878-Pregnancy,
pubmed-meshheading:19470878-Pregnancy in Diabetics,
pubmed-meshheading:19470878-RNA, Messenger,
pubmed-meshheading:19470878-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:19470878-Time Factors,
pubmed-meshheading:19470878-Tissue Culture Techniques,
pubmed-meshheading:19470878-Young Adult
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| pubmed:year |
2009
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| pubmed:articleTitle |
Circulating and placental growth-differentiation factor 15 in preeclampsia and in pregnancy complicated by diabetes mellitus.
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| pubmed:affiliation |
Department of Obstetrics and Gynaecology, Oslo University Hospital, University of Oslo, Norway.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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