Linked Life Data

19469563

Source:http://linkedlifedata.com/resource/pubmed/id/19469563

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
24
pubmed:dateCreated
2009-6-11
pubmed:abstractText
For the first time, the covalent immobilization of oligonucleotides double helices onto surfaces prior to sequential denaturation and rehybridization is proven to lead to optimal hybridization efficiency. Two indirect methods were used for monitoring these reactions in situ: the quartz crystal microbalance with dissipation monitoring (QCM-D) and the wavelength interrogated optical sensor (WIOS, Bright Reader). Both techniques led to the result that with this immobilization approach one could reach nearly 100% hybridization efficiency. Moreover, applying the polymer theory to the adsorption of nucleotide sequences onto surfaces, we demonstrate, that for single stranded sequences the coil conformation prevails over the stretch one.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1520-6106
pubmed:author
pubmed:issnType
Print
pubmed:day
18
pubmed:volume
113
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8383-90
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Optimal hybridization efficiency upon immobilization of oligonucleotide double helices.
pubmed:affiliation
Department of Chemistry, University of Basel, Klingelbergstrasse 80, CH-4056, Basel, Switzerland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't