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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
22
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pubmed:dateCreated |
1991-12-26
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pubmed:abstractText |
The role of major histocompatibility complex (MHC) class I expression in control of the sensitivity of normal cells to natural killer (NK) cells was studied by the use of mutant mice made deficient for expression of beta 2-microglobulin (beta 2m) through homologous recombination in embryonal stem cells. T-cell blasts from beta 2m-deficient (beta 2m -/-) mice were killed by NK cells from normal mice in vitro, while beta 2m +/- blasts were resistant. The beta 2m defect also affected the NK effector cell repertoire: NK cells from beta 2m -/- mice failed to kill beta 2m -/- blasts, while they retained the ability to kill the prototype NK cell target lymphoma YAC-1, although at reduced levels. The inability to recognize beta 2m -/- blasts could be transferred with beta 2m -/- bone marrow to irradiated beta 2m-expressing mice. In contrast, the development of CD8+ T cells (deficient in beta 2m -/- mice) was restored in such chimera. These results indicate that loss of MHC class I/beta 2m expression is sufficient to render normal cells sensitive to NK cells, and that the same defect in the hemopoietic system of a mouse renders its NK cells tolerant to beta 2m-deficient but otherwise normal cells. In the beta 2m -/- mice, NK cells may be selected or educated by other bone marrow cells to tolerate the MHC class I deficiency. Alternatively, the specificity may be controlled directly by the class I molecules on the NK cells themselves.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946452-1694227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946452-1825103,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946452-1853205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946452-1856626,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946452-1979875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946452-1987491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946452-2021423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946452-2112266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946452-2113557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946452-2139497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946452-2201309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946452-2654290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946452-2664068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946452-2682666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946452-2685607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946452-2814488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946452-2970593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946452-3171481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946452-3549904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946452-3584981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946452-3951539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946452-4942407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946452-593386
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
88
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10332-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1946452-Animals,
pubmed-meshheading:1946452-Antibodies, Monoclonal,
pubmed-meshheading:1946452-Bone Marrow,
pubmed-meshheading:1946452-Cell Line,
pubmed-meshheading:1946452-Chimera,
pubmed-meshheading:1946452-Concanavalin A,
pubmed-meshheading:1946452-Cytotoxicity, Immunologic,
pubmed-meshheading:1946452-Flow Cytometry,
pubmed-meshheading:1946452-Killer Cells, Natural,
pubmed-meshheading:1946452-Lymphocyte Activation,
pubmed-meshheading:1946452-Mice,
pubmed-meshheading:1946452-Mice, Inbred C57BL,
pubmed-meshheading:1946452-Phenotype,
pubmed-meshheading:1946452-Plant Lectins,
pubmed-meshheading:1946452-Spleen,
pubmed-meshheading:1946452-T-Lymphocytes,
pubmed-meshheading:1946452-beta 2-Microglobulin
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pubmed:year |
1991
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pubmed:articleTitle |
Recognition of beta 2-microglobulin-negative (beta 2m-) T-cell blasts by natural killer cells from normal but not from beta 2m- mice: nonresponsiveness controlled by beta 2m- bone marrow in chimeric mice.
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pubmed:affiliation |
Department of Tumor Biology, Karolinska Institute, Stockholm, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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