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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
22
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pubmed:dateCreated |
1991-12-26
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pubmed:abstractText |
A clone for the iron-responsive element (IRE)-binding protein (IRE-BP) has been transfected and expressed in mouse fibroblasts. The IRE-BP gene product binds IREs with high affinity and specificity. Amino acid alignments reveal that the IRE-BP is 30% identical to mitochondrial aconitase. The 18 active site residues of mitochondrial aconitase are identical to those in the IRE-BP, suggesting that the IRE-BP may possess aconitase activity. After purification of native IRE-BP and immunoaffinity purification of transfected and expressed IRE-BP, we demonstrate that the purified IRE-BP has aconitase activity.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946430-196813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946430-2001588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946430-2172968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946430-2303429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946430-2474819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946430-2557832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946430-2598939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946430-2601708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946430-2683086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946430-2726740,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946430-2771641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946430-2798408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946430-2827008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946430-2836270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946430-2997226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946430-3127826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946430-3162307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946430-3402439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946430-3413484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946430-3470792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946430-3685996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946430-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946430-6316339
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
88
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10109-13
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1946430-Aconitate Hydratase,
pubmed-meshheading:1946430-Amino Acid Sequence,
pubmed-meshheading:1946430-Animals,
pubmed-meshheading:1946430-Cells, Cultured,
pubmed-meshheading:1946430-Chimera,
pubmed-meshheading:1946430-Cloning, Molecular,
pubmed-meshheading:1946430-DNA,
pubmed-meshheading:1946430-Ferritins,
pubmed-meshheading:1946430-Genes, myc,
pubmed-meshheading:1946430-Humans,
pubmed-meshheading:1946430-Iron-Regulatory Proteins,
pubmed-meshheading:1946430-Mice,
pubmed-meshheading:1946430-Molecular Sequence Data,
pubmed-meshheading:1946430-Open Reading Frames,
pubmed-meshheading:1946430-RNA-Binding Proteins,
pubmed-meshheading:1946430-Restriction Mapping,
pubmed-meshheading:1946430-Transcription, Genetic,
pubmed-meshheading:1946430-Transfection
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pubmed:year |
1991
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pubmed:articleTitle |
A regulated RNA binding protein also possesses aconitase activity.
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pubmed:affiliation |
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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