Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
1991-12-26
pubmed:abstractText
The x-ray structure of a short-chain dehydrogenase, the bacterial holo 3 alpha,20 beta-hydroxysteroid dehydrogenase (EC 1.1.1.53), is described at 2.6 A resolution. This enzyme is active as a tetramer and crystallizes with four identical subunits in the asymmetric unit. It has the alpha/beta fold characteristic of the dinucleotide binding region. The fold of the rest of the subunit, the quaternary structure, and the nature of the cofactor-enzyme interactions are, however, significantly different from those observed in the long-chain dehydrogenases. The architecture of the postulated active site is consistent with the observed stereospecificity of the enzyme and the fact that the tetramer is the active form. There is only one cofactor and one substrate-binding site per subunit; the specificity for both 3 alpha- and 20 beta-ends of the steroid results from the binding of the steroid in two orientations near the same cofactor at the same catalytic site.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1946424-178875, http://linkedlifedata.com/resource/pubmed/commentcorrection/1946424-2051487, http://linkedlifedata.com/resource/pubmed/commentcorrection/1946424-2194840, http://linkedlifedata.com/resource/pubmed/commentcorrection/1946424-2296288, http://linkedlifedata.com/resource/pubmed/commentcorrection/1946424-2330370, http://linkedlifedata.com/resource/pubmed/commentcorrection/1946424-2337593, http://linkedlifedata.com/resource/pubmed/commentcorrection/1946424-2351678, http://linkedlifedata.com/resource/pubmed/commentcorrection/1946424-2495285, http://linkedlifedata.com/resource/pubmed/commentcorrection/1946424-2808402, http://linkedlifedata.com/resource/pubmed/commentcorrection/1946424-3020515, http://linkedlifedata.com/resource/pubmed/commentcorrection/1946424-3105578, http://linkedlifedata.com/resource/pubmed/commentcorrection/1946424-3455925, http://linkedlifedata.com/resource/pubmed/commentcorrection/1946424-35535, http://linkedlifedata.com/resource/pubmed/commentcorrection/1946424-3586018, http://linkedlifedata.com/resource/pubmed/commentcorrection/1946424-4302996, http://linkedlifedata.com/resource/pubmed/commentcorrection/1946424-4357832, http://linkedlifedata.com/resource/pubmed/commentcorrection/1946424-4366080, http://linkedlifedata.com/resource/pubmed/commentcorrection/1946424-4618777, http://linkedlifedata.com/resource/pubmed/commentcorrection/1946424-4797043, http://linkedlifedata.com/resource/pubmed/commentcorrection/1946424-6098306, http://linkedlifedata.com/resource/pubmed/commentcorrection/1946424-6581837, http://linkedlifedata.com/resource/pubmed/commentcorrection/1946424-6746641, http://linkedlifedata.com/resource/pubmed/commentcorrection/1946424-6789320, http://linkedlifedata.com/resource/pubmed/commentcorrection/1946424-6855599, http://linkedlifedata.com/resource/pubmed/commentcorrection/1946424-6928375, http://linkedlifedata.com/resource/pubmed/commentcorrection/1946424-7020376, http://linkedlifedata.com/resource/pubmed/commentcorrection/1946424-7338899, http://linkedlifedata.com/resource/pubmed/commentcorrection/1946424-7411626
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
88
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
10064-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Three-dimensional structure of holo 3 alpha,20 beta-hydroxysteroid dehydrogenase: a member of a short-chain dehydrogenase family.
pubmed:affiliation
Medical Foundation of Buffalo, NY 14203.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.