Linked Life Data

19463842

Source:http://linkedlifedata.com/resource/pubmed/id/19463842

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2009-7-20
pubmed:abstractText
A Gloydius blomhoffi brevicaudus venom gland cDNA library was screened to isolate cDNA clones using probes based on highly conserved amino acid sequences from known ecto-5'-nucleotidases (ecto-5'-NTs). Molecular cloning of ecto-5'-NT from G. blomhoffi brevicaudus venom predicted that it was a glycosyl phosphatidylinositol-anchored membrane protein containing 588 amino acid residues with 7 potential N-linked glycosylation sites. The deduced amino acid sequence shows approximately 60% sequence identity to mammalian ecto-5'-NT sequences. This is the first report of the primary structure of ecto-5'-NT from a reptile. Gel-filtration chromatography of fresh venom from Gloydius blomhoffi blomhoffi, a subspecies of G. blomhoffi, revealed that at least a part of ecto-5'-NT is bound to exosome-like vesicles.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1879-3150
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
54
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
408-12
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Molecular cloning and characterization of ecto-5'-nucleotidase from the venoms of Gloydius blomhoffi.
pubmed:affiliation
Institute of Medicinal Chemistry, Hoshi University, Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
pubmed:publicationType
Journal Article