Linked Life Data

19462052

Source:http://linkedlifedata.com/resource/pubmed/id/19462052

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2009-5-22
pubmed:abstractText
Pathological amyloid deposits are mixtures of polypeptides and non-proteinaceous species including heparan sulfate proteoglycans and glycosaminoglycans (GAGs). We describe a procedure in which a (13)C-labelled N-acetyl derivative of the GAG heparin ([(13)C-CH(3)]NAcHep) serves as a useful probe for the analysis of GAG-protein interactions in amyloid using solid-state nuclear magnetic resonance (SSNMR) spectroscopy. NAcHep emulates heparin by enhancing aggregation and altering the fibril morphology of Abeta(1-40), one of the beta-amyloid polypeptides associated with Alzheimer's disease, and alpha-synuclein, the major protein component of Lewy bodies associated with Parkinson's disease. (13)C SSNMR spectra confirm the presence of [(13)C-CH(3)]NAcHep in Abeta(1-40) fibril deposits and detect dipolar couplings between the glycan and arginine R(5) at the Abeta(1-40) N-terminus, suggesting that the two species are intimately mixed at the molecular level. This procedure provides a foundation for further extensive investigations of polypeptide-glycan interactions within amyloid fibrils.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1477-0539
pubmed:author
pubmed:issnType
Electronic
pubmed:day
7
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2414-20
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Exploiting a (13)C-labelled heparin analogue for in situ solid-state NMR investigations of peptide-glycan interactions within amyloid fibrils.
pubmed:affiliation
School of Biological Sciences, University of Liverpool, Crown Street, Liverpool, L69 7ZB, United Kingdom.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't