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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2009-5-22
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pubmed:abstractText |
The present study was conducted to examine post-thaw in vitro developmental competence of buffalo embryos cryopreserved by cytoskeletal stabilization and vitrification. In vitro produced embryos were incubated with a medium containing cytochalasin-b (cyto-b) in a CO(2) incubator for 40 min for microfilament stabilization and were cryopreserved by a two-step vitrification method at 24 degrees C in the presence of cyto-b. Initially, the embryos were exposed to 10% ethylene glycol (EG) and 10% dimethylsulfoxide (DMSO) in a base medium for 4 min. After the initial exposure, the embryos were transferred to a 7 microl drop of 25% EG and 25% DMSO in base medium and 0.3 M sucrose for 45 sec. After warming, the embryos were cultured in vitro for 72 h. The post-thaw in vitro developmental competence of the cyto-b-treated embryos did not differ significantly from those vitrified without cyto-b treatment. The hatching rates of morulae vitrified without cyto-b treatment was significantly lower than the non-vitrified control. However, the hatching rate of cyto-b-treated vitrified morulae did not differ significantly from the non-vitrified control. This study demonstrates that freezing of buffalo embryos by cytoskeletal stabilization and vitrification is a reliable method for long-term preservation.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19461211-10413573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19461211-10684796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19461211-10700641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19461211-10735132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19461211-11775973,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19461211-15036974,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19461211-16169072,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19461211-16727625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19461211-17689038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19461211-17933564,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19461211-3652721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19461211-3969158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19461211-6709062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19461211-6857250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19461211-6864181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19461211-7310267,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19461211-8107029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19461211-8204105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19461211-9728813
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1976-555X
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
153-6
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:19461211-Actin Cytoskeleton,
pubmed-meshheading:19461211-Animals,
pubmed-meshheading:19461211-Buffaloes,
pubmed-meshheading:19461211-Cryopreservation,
pubmed-meshheading:19461211-Cryoprotective Agents,
pubmed-meshheading:19461211-Cytochalasin B,
pubmed-meshheading:19461211-Dimethyl Sulfoxide,
pubmed-meshheading:19461211-Embryo Culture Techniques,
pubmed-meshheading:19461211-Ethylene Glycol,
pubmed-meshheading:19461211-Female,
pubmed-meshheading:19461211-Fertilization in Vitro,
pubmed-meshheading:19461211-Male,
pubmed-meshheading:19461211-Pregnancy
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pubmed:year |
2009
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pubmed:articleTitle |
Post-thaw development of in vitro produced buffalo embryos cryopreserved by cytoskeletal stabilization and vitrification.
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pubmed:affiliation |
Department of Animal Reproduction, Veterinary College, Karnataka Veterinary, Animal and Fishery Sciences University, Bangalore, India. drmanjunathvet@gmail.com
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pubmed:publicationType |
Journal Article
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