Linked Life Data

19460430

Source:http://linkedlifedata.com/resource/pubmed/id/19460430

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2010-2-3
pubmed:abstractText
Hazard information essential to guide developmental neurotoxicity risk assessments is limited for many chemicals. As developmental neurotoxicity testing using rodents is laborious and expensive, alternative species such as zebrafish are being adapted for rapid toxicity screening. Assessing the developmental neurotoxicity potential of chemicals in a rapid throughput mode will be aided by the identification and characterization of transcriptional biomarkers that can be measured accurately and rapidly. To this end, the developmental expression profiles of ten nervous system genes were characterized in 1 to 6 days post fertilization zebrafish embryos/larvae using quantitative real time PCR (qRT-PCR). Transcripts of synapsinII a (syn2a) and myelin basic protein (mbp) increased throughout development, while transcripts of gap43, elavl3, nkx2.2a, neurogenin1 (ngn1), alpha1-tubulin, and glial fibrillary acidic protein (gfap) initially increased, but subsequently declined. Transcripts for nestin and sonic hedgehog a (shha) decreased during development. We tested the responses of these potential biomarkers to developmental neurotoxicant exposure, and found that the expression profiles of a subset of genes were altered both during and after exposure to sublethal doses of ethanol, a known developmental neurotoxicant. Collectively, these data indicate that transcript levels of the candidate genes change during development in patterns which are consistent with literature reports, and that the expression of the transcripts is perturbed by treatment with a developmental neurotoxicant (ethanol). These results suggest that the expression profiles of these genes may be useful biomarkers for rapid evaluation of the developmental neurotoxicity potential of chemicals.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1872-9738
pubmed:author
pubmed:copyrightInfo
Published by Elsevier Inc.
pubmed:issnType
Electronic
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
91-8
pubmed:meshHeading
pubmed:articleTitle
Gene expression changes in developing zebrafish as potential markers for rapid developmental neurotoxicity screening.
pubmed:affiliation
Cellular and Molecular Toxicology Branch, Neurotoxicology Division, United States Environmental Protection Agency, Research Triangle Park, NC 27711, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.