Linked Life Data

19459622

Source:http://linkedlifedata.com/resource/pubmed/id/19459622

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2009-6-23
pubmed:abstractText
Water-soluble single-walled nanotubes (SWNTs) are being tested as contrast agents for medical imaging and for the delivery of therapeutically active molecules to target cells. However, before they become used commercially, it will be essential to establish their subcellular distribution and whether they are cytotoxic. Here we characterize uptake of unlabeled, acid-treated, water-soluble SWNTs by human monocyte derived macrophage cells using a combination of Raman spectroscopy and analytical electron microscopy and compare our findings to previous work on unpurified SWNTs. Raman spectroscopy demonstrated that acid-treated SWNTs had a greater number of functional groups on the carbon walls than nontreated SWNT. The acid-treated SWNTs were less aggregated within cells than unpurified SWNTs. Bundles, and also individual acid-treated SWNTs, were found frequently inside lysosomes and also the cytoplasm, where they caused no significant changes in cell viability or structure even after 4 days of exposure.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1936-086X
pubmed:author
pubmed:issnType
Electronic
pubmed:day
23
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1485-92
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Uptake of noncytotoxic acid-treated single-walled carbon nanotubes into the cytoplasm of human macrophage cells.
pubmed:affiliation
Department of Materials, Imperial College London, South Kensington, London, United Kingdom. a.porter@imperial.ac.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't