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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2009-8-5
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pubmed:abstractText |
The catalytic turnover of cytochrome P450( cam ) from Pseudomonas putida requires two auxiliary reduction partners, putidaredoxin (Pd) and putidaredoxin reductase (PdR). We report the functional expression in Escherichia coli of tricistronic constructs consisting of P450( cam ) encoded by the first cistron and the auxiliary proteins, Pd and PdR by the second and the third. Transformed bacterial whole cells efficiently oxidized (1R)-(+)-camphor to 5-exo-hydroxycamphor and, interestingly, limonene to (-)-perillyl alcohol. These bioengineered E. coli cells possess a heterologous self-sufficient P450 catalytic system that may have advantages in terms of low cost and high yield for the production of fine chemicals.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Camphor,
http://linkedlifedata.com/resource/pubmed/chemical/Camphor 5-Monooxygenase,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclohexenes,
http://linkedlifedata.com/resource/pubmed/chemical/Ferredoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Monoterpenes,
http://linkedlifedata.com/resource/pubmed/chemical/NADH, NADPH Oxidoreductases,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Terpenes,
http://linkedlifedata.com/resource/pubmed/chemical/limonene,
http://linkedlifedata.com/resource/pubmed/chemical/perilla alcohol,
http://linkedlifedata.com/resource/pubmed/chemical/putidaredoxin,
http://linkedlifedata.com/resource/pubmed/chemical/putidaredoxin reductase
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1573-6776
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
31
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1427-31
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pubmed:dateRevised |
2011-4-14
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pubmed:meshHeading |
pubmed-meshheading:19458919-Biotransformation,
pubmed-meshheading:19458919-Camphor,
pubmed-meshheading:19458919-Camphor 5-Monooxygenase,
pubmed-meshheading:19458919-Cyclohexenes,
pubmed-meshheading:19458919-Escherichia coli,
pubmed-meshheading:19458919-Ferredoxins,
pubmed-meshheading:19458919-Gene Dosage,
pubmed-meshheading:19458919-Gene Expression,
pubmed-meshheading:19458919-Monoterpenes,
pubmed-meshheading:19458919-NADH, NADPH Oxidoreductases,
pubmed-meshheading:19458919-Pseudomonas putida,
pubmed-meshheading:19458919-Recombinant Proteins,
pubmed-meshheading:19458919-Terpenes
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pubmed:year |
2009
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pubmed:articleTitle |
Tricistronic overexpression of cytochrome P450cam, putidaredoxin, and putidaredoxin reductase provides a useful cell-based catalytic system.
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pubmed:affiliation |
Department of Biological Sciences, Konkuk University, 1 Hwayang-dong, Gwangjn-gu, Seoul 143-701, Korea. donghak@konkuk.ac.kr
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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