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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2009-7-21
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pubmed:abstractText |
The lectin Siglec-8 (sialic acid-binding, immunoglobulin-like lectin), which is selectively expressed on eosinophil surfaces and regulates eosinophil survival, preferentially binds to the glycan 6'-sulfo-sialyl Lewis X (6'-sulfo-sLe(x)). Antibody engagement of Siglec-8 on eosinophils causes their apoptosis, suggesting that engagement of Siglec-8 with its natural glycan ligands in vivo may control allergic inflammation. We report that a soluble synthetic polymer displaying 6'-sulfo-sLe(x) glycan selectively binds to human eosinophils and human embryonic kidney 293 cells expressing Siglec-8. Binding was inhibited by anti-Siglec-8 antibody. In whole blood, eosinophils were the only leukocyte subtype to detectably bind polymeric 6'-sulfo-sLe(x). Interleukin-5-primed eosinophils underwent apoptosis when incubated with either anti-Siglec-8 monoclonal antibody or polymeric 6'-sulfo-sLe(x), although the glycan polymer was less effective. These data demonstrate that a soluble, multivalent glycan selectively binds to human eosinophils and induces their apoptosis in vitro and provide proof-of-concept that such a reagent could be used to selectively target eosinophils.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19458105-10567377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19458105-10625619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19458105-10764831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19458105-10856141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19458105-12609831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19458105-15563466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19458105-15864434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19458105-15972893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19458105-16118287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19458105-16157303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19458105-16732727,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19458105-17272508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19458105-17337295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19458105-17380156,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19458105-17562860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19458105-17690326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19458105-18036650,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19458105-18579791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19458105-18699932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19458105-19076345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19458105-19135419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19458105-19178537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19458105-7506734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19458105-7543306,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19458105-8400823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19458105-9155832
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Apoptosis Regulatory Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Inflammation Mediators,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Oligosaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/SIGLEC8 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/sialyl-2-3-(6'-sulfo)galactosyl-1-4-...
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1521-0103
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
330
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
608-12
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pubmed:dateRevised |
2011-3-2
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pubmed:meshHeading |
pubmed-meshheading:19458105-Antigens, CD,
pubmed-meshheading:19458105-Antigens, Differentiation, B-Lymphocyte,
pubmed-meshheading:19458105-Apoptosis,
pubmed-meshheading:19458105-Apoptosis Regulatory Proteins,
pubmed-meshheading:19458105-Cell Line,
pubmed-meshheading:19458105-Eosinophils,
pubmed-meshheading:19458105-Humans,
pubmed-meshheading:19458105-Inflammation Mediators,
pubmed-meshheading:19458105-Lectins,
pubmed-meshheading:19458105-Ligands,
pubmed-meshheading:19458105-Oligosaccharides,
pubmed-meshheading:19458105-Protein Binding
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pubmed:year |
2009
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pubmed:articleTitle |
Eosinophil-selective binding and proapoptotic effect in vitro of a synthetic Siglec-8 ligand, polymeric 6'-sulfated sialyl Lewis x.
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pubmed:affiliation |
Division of Allergy and Clinical Immunology, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA. bbochner@jhmi.edu.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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