Linked Life Data

19455339

Source:http://linkedlifedata.com/resource/pubmed/id/19455339

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2009-6-26
pubmed:abstractText
Traditional transformation methods are complex and time consuming. It is generally difficult to transform indica rice varieties using traditional transformation methods due to their poor regeneration. In this contribution, a simple method was developed for the transformation of indica rice. In this method, the mature embryos of soaked seeds were pierced by a needle, and then soaked in the Agrobacterium inoculum under vacuum infiltration. The inoculated seeds germinated and grew to maturation (T (0)) under nonsterile conditions. The herbicide or antibiotic analysis and molecular analysis were conducted on T (0) plants. The results showed that although the efficiency of transformation was about 6.0%, it was easier to transform indica rice using the proposed method, and the transformation process was significantly shortened. The success of transformation was further confirmed by the genetic and molecular analyses of T (1) transformants.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1432-203X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1065-74
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Piercing and vacuum infiltration of the mature embryo: a simplified method for Agrobacterium-mediated transformation of indica rice.
pubmed:affiliation
Institute of Bioscience and Biotechnology, Hunan University, Changsha, 410082, Hunan, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't