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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2009-5-20
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pubmed:abstractText |
In a humid milieu such as mucosal surfaces, pollen grains do not only release allergens but also proinflammatory and immunomodulatory lipids, termed pollen-associated lipid mediators. Among these, the E(1)-phytoprostanes (PPE(1)) were identified to modulate dendritic cell (DC) function: PPE(1) inhibit the DC's capacity to produce IL-12 and enhance DC mediated T(H)2 polarization of naive T cells. The mechanism(s) by which PPE(1) act on DC remained elusive. We thus analyzed candidate signaling elements and their role in PPE(1)-mediated regulation of DC function. Aqueous birch pollen extracts induced a marked cAMP response in DC that could be blocked partially by EP2 and EP4 antagonists. In contrast, PPE(1) hardly induced cAMP and the inhibitory effect on IL-12 production was mostly independent of EP2 and EP4. Instead, PPE(1) inhibited the LPS-induced production of IL-12 p70 by a mechanism involving the nuclear receptor PPAR-gamma. Finally, PPE(1) efficiently blocked NF-kappaB signaling in DCs by inhibiting IkappaB-alpha degradation, translocation of p65 to the nucleus, and binding to its target DNA elements. We conclude that pollen-derived PPE(1) modulate DC function via PPAR-gamma dependent pathways that lead to inhibition of NFkappaB activation and result in reduced DC IL-12 production and consecutive T(H)2 polarization.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclopentanes,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acids, Unsaturated,
http://linkedlifedata.com/resource/pubmed/chemical/Immunologic Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/PPAR gamma,
http://linkedlifedata.com/resource/pubmed/chemical/PTGER2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/PTGER4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Prostaglandin E,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Prostaglandin E, EP2...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Prostaglandin E, EP4...
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1550-6606
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
182
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6653-8
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:19454659-Adolescent,
pubmed-meshheading:19454659-Adult,
pubmed-meshheading:19454659-Betula,
pubmed-meshheading:19454659-Cells, Cultured,
pubmed-meshheading:19454659-Cyclic AMP,
pubmed-meshheading:19454659-Cyclopentanes,
pubmed-meshheading:19454659-Dendritic Cells,
pubmed-meshheading:19454659-Fatty Acids, Unsaturated,
pubmed-meshheading:19454659-Humans,
pubmed-meshheading:19454659-Immunologic Factors,
pubmed-meshheading:19454659-Interleukin-12,
pubmed-meshheading:19454659-Middle Aged,
pubmed-meshheading:19454659-NF-kappa B,
pubmed-meshheading:19454659-PPAR gamma,
pubmed-meshheading:19454659-Pollen,
pubmed-meshheading:19454659-Receptors, Prostaglandin E,
pubmed-meshheading:19454659-Receptors, Prostaglandin E, EP2 Subtype,
pubmed-meshheading:19454659-Receptors, Prostaglandin E, EP4 Subtype,
pubmed-meshheading:19454659-Signal Transduction,
pubmed-meshheading:19454659-Th2 Cells,
pubmed-meshheading:19454659-Young Adult
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pubmed:year |
2009
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pubmed:articleTitle |
Pollen-derived E1-phytoprostanes signal via PPAR-gamma and NF-kappaB-dependent mechanisms.
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pubmed:affiliation |
Division of Environmental Dermatology and Allergy Helmholz Center Munich, Zentrum Allergie und Umwelt, Technische Universität Munich, Munich, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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