Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2009-10-30
pubmed:abstractText
A procedure has been developed for directly depositing membrane fragments derived from bacterial cells (chromatophores from Rhodopseudomonas sphaeroides) and mammalian cells (mu-opioid receptor- and MC4 receptor-transfected human embryonic kidney (HEK) cells and rat trigeminal ganglion cells) on the silica surface of a plasmon-waveguide resonance (PWR) spectrometer. Binding of ligands (cytochrome c(2) for the chromatophores, the peptide agonists DAMGO and melanotan-II that are specific for the mu-opioid and MC4 receptors, and two nonpeptide agonists that are specific for the CB1 receptor) to these membrane fragments has been observed and characterized with high sensitivity using PWR spectral shifts. The K(D) values obtained are in excellent agreement with conventional pharmacological assays and with prior PWR studies using purified receptors inserted into deposited lipid bilayer membranes. These studies provide a new tool for obtaining useful biological information about receptor-mediated processes in real biological membranes.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19454250-10366845, http://linkedlifedata.com/resource/pubmed/commentcorrection/19454250-10990964, http://linkedlifedata.com/resource/pubmed/commentcorrection/19454250-11053123, http://linkedlifedata.com/resource/pubmed/commentcorrection/19454250-11106620, http://linkedlifedata.com/resource/pubmed/commentcorrection/19454250-11222316, http://linkedlifedata.com/resource/pubmed/commentcorrection/19454250-12496104, http://linkedlifedata.com/resource/pubmed/commentcorrection/19454250-12849749, http://linkedlifedata.com/resource/pubmed/commentcorrection/19454250-14581204, http://linkedlifedata.com/resource/pubmed/commentcorrection/19454250-1478950, http://linkedlifedata.com/resource/pubmed/commentcorrection/19454250-15009533, http://linkedlifedata.com/resource/pubmed/commentcorrection/19454250-15610035, http://linkedlifedata.com/resource/pubmed/commentcorrection/19454250-15668234, http://linkedlifedata.com/resource/pubmed/commentcorrection/19454250-15771429, http://linkedlifedata.com/resource/pubmed/commentcorrection/19454250-16766609, http://linkedlifedata.com/resource/pubmed/commentcorrection/19454250-17516628, http://linkedlifedata.com/resource/pubmed/commentcorrection/19454250-18162180, http://linkedlifedata.com/resource/pubmed/commentcorrection/19454250-1850617, http://linkedlifedata.com/resource/pubmed/commentcorrection/19454250-18615441, http://linkedlifedata.com/resource/pubmed/commentcorrection/19454250-2941079, http://linkedlifedata.com/resource/pubmed/commentcorrection/19454250-6334313, http://linkedlifedata.com/resource/pubmed/commentcorrection/19454250-8114680, http://linkedlifedata.com/resource/pubmed/commentcorrection/19454250-8534815, http://linkedlifedata.com/resource/pubmed/commentcorrection/19454250-9370473, http://linkedlifedata.com/resource/pubmed/commentcorrection/19454250-9622672, http://linkedlifedata.com/resource/pubmed/commentcorrection/19454250-9726940, http://linkedlifedata.com/resource/pubmed/commentcorrection/19454250-9876164
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1096-0309
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
387
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
95-101
pubmed:dateRevised
2011-9-26
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Plasmon-waveguide resonance studies of ligand binding to integral proteins in membrane fragments derived from bacterial and mammalian cells.
pubmed:affiliation
Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, AZ 85721, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural