Linked Life Data

19453171

Source:http://linkedlifedata.com/resource/pubmed/id/19453171

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2009-6-17
pubmed:abstractText
Alkylresorcinols (AR) present in the bran fraction of wheat, rye, and barley grains are of current interest as biomarkers of wholegrain cereal intake. This paper reports the development of a rapid quantitative HPLC method allowing sensitive analysis of AR in cereals in 14 min using coularray (CA) detection. The LOD was 1 ng injected and the LOQ 2.5 ng injected, whereas the intrarun and inter-run CVs were 0.8 and 6.4%, respectively. Analytical recovery determined by spiking ranged from 98 to 107% for AR homologues C15:0-C25:0. The method was used to analyze 79 flour and cereal products, with results in the same range as previous studies using GC analysis. Analysis of wholegrain white wheat were in the same range as other wheat varieties (420-556 microg/g). Use of the CA detector allowed detection of low concentrations of AR in white wheat flour (13-51 microg/g) that had not been reliably detected using previous methods. This method allows rapid throughput analysis of cereal samples, required for validation studies on the use of AR as biomarkers of wholegrain cereal intake.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1520-5118
pubmed:author
pubmed:issnType
Electronic
pubmed:day
24
pubmed:volume
57
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5187-93
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Rapid and sensitive analysis of alkylresorcinols from cereal grains and products using HPLC-Coularray-based electrochemical detection.
pubmed:affiliation
Department of Bioanalytical Sciences, Nestle Research Centre, Lausanne 26, Swizterland. Alastair.Ross@rdls.nestle.com
pubmed:publicationType
Journal Article, Evaluation Studies