rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
2
|
pubmed:dateCreated |
2009-6-9
|
pubmed:abstractText |
Chronic hepatitis C virus (HCV) infection is a worldwide public issue. In this study, we performed bioactivity-guided screening of the Lonicera hypoglauca Miq. crude extracts to find for naturally chemical entities with anti-HCV activity. Pheophytin a was identified from the ethanol-soluble fraction of L. hypoglauca that elicited dose-dependent inhibition of HCV viral proteins and RNA expression in both replicon cells and cell culture infectious system. Computational modeling revealed that pheophytin a can bind to the active site of HCV-NS3, suggesting that NS3 is a potent molecular target of pheophytin a. Biochemical analysis further revealed that pheophytin a inhibited NS3 serine protease activity with IC(50)=0.89 microM. Notably, pheophytin a and IFNalpha-2a elicited synergistic anti-HCV activity in replicon cells with no significant cytotoxicity. This study thereby demonstrates for the first time that pheophytin a is a potent HCV-NS3 protease inhibitor and offers insight for development of novel anti-HCV regimens.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Jul
|
pubmed:issn |
1090-2104
|
pubmed:author |
|
pubmed:issnType |
Electronic
|
pubmed:day |
24
|
pubmed:volume |
385
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
230-5
|
pubmed:dateRevised |
2011-11-17
|
pubmed:meshHeading |
pubmed-meshheading:19450556-Antiviral Agents,
pubmed-meshheading:19450556-Catalytic Domain,
pubmed-meshheading:19450556-Cell Line,
pubmed-meshheading:19450556-Computer Simulation,
pubmed-meshheading:19450556-Drug Evaluation, Preclinical,
pubmed-meshheading:19450556-Hepacivirus,
pubmed-meshheading:19450556-Humans,
pubmed-meshheading:19450556-Interferon-alpha,
pubmed-meshheading:19450556-Lonicera,
pubmed-meshheading:19450556-Models, Molecular,
pubmed-meshheading:19450556-Pheophytins,
pubmed-meshheading:19450556-Recombinant Proteins,
pubmed-meshheading:19450556-Viral Nonstructural Proteins,
pubmed-meshheading:19450556-Viral Proteins,
pubmed-meshheading:19450556-Virus Replication
|
pubmed:year |
2009
|
pubmed:articleTitle |
Bioactivity-guided screening identifies pheophytin a as a potent anti-hepatitis C virus compound from Lonicera hypoglauca Miq.
|
pubmed:affiliation |
Department of Forestry, National Chung Hsing University, Taichung, Taiwan, ROC.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|