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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2010-2-1
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pubmed:abstractText |
Analysis of molecular genetic markers in biological fluids has been proposed as a useful tool for cancer diagnosis. MicroRNAs (miRNAs) are small regulatory RNAs that are frequently dysregulated in lung cancer and have shown promise as tissue-based markers for its prognostication. The aim of this study was to determine whether aberrant miRNA expression can be used as a marker in sputum specimen for the diagnosis of non-small cell lung cancer (NSCLC). Experimental design: expressions of mature miRNAs, mir-21 and mir-155, were examined by real-time reverse transcription polymerase chain reaction (RT-PCR) and normalized to that of control miRNA, U6B, in sputum of 23 patients with NSCLC and 17 cancer-free subjects. The data was compared with conventional sputum cytology for the diagnosis of lung cancer. All endogenous miRNAs were present in sputum in a remarkably stable form and sensitively and specifically detected by real-time RT-PCR. Mir-21 expression in the sputum specimens was significantly higher in cancer patients (76.32+/-9.79) than cancer-free individuals (62.24+/-3.82) (P<0.0001). Furthermore, overexpression of mir-21 showed highly discriminative receiver-operator characteristic (ROC) curve profile, clearly distinguishing cancer patients from cancer-free subjects with areas under the ROC curve at 0.902+/-0.054. Detection of mir-21 expression produced 69.66% sensitivity and 100.00% specificity in diagnosis of lung cancer, as compared with 47.82% sensitivity and 100.00% specificity by sputum cytology. The measurement of altered miRNA expression in sputum could be a useful noninvasive approach for the diagnosis of lung cancer.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19446359-11205917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19446359-11577478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19446359-11679670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19446359-12086887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19446359-12527573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19446359-14600122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19446359-15172979,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19446359-16024602,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19446359-16314309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19446359-16530703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19446359-17255269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19446359-17911264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19446359-18270258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19446359-18460397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19446359-18484646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19446359-18663219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19446359-18719201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19446359-19275611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19446359-4810100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19446359-6091505
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1872-8332
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2009 Elsevier Ireland Ltd. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:volume |
67
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
170-6
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pubmed:dateRevised |
2011-7-22
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pubmed:meshHeading |
pubmed-meshheading:19446359-Aged,
pubmed-meshheading:19446359-Area Under Curve,
pubmed-meshheading:19446359-Carcinoma, Non-Small-Cell Lung,
pubmed-meshheading:19446359-Female,
pubmed-meshheading:19446359-Humans,
pubmed-meshheading:19446359-Lung Neoplasms,
pubmed-meshheading:19446359-Male,
pubmed-meshheading:19446359-MicroRNAs,
pubmed-meshheading:19446359-Neoplasm Staging,
pubmed-meshheading:19446359-RNA Stability,
pubmed-meshheading:19446359-ROC Curve,
pubmed-meshheading:19446359-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:19446359-Sensitivity and Specificity,
pubmed-meshheading:19446359-Sputum,
pubmed-meshheading:19446359-Tumor Markers, Biological
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pubmed:year |
2010
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pubmed:articleTitle |
Altered miRNA expression in sputum for diagnosis of non-small cell lung cancer.
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pubmed:affiliation |
Department of Pathology, University of Maryland School of Medicine, Baltimore, MD, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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