Linked Life Data

19446299

Source:http://linkedlifedata.com/resource/pubmed/id/19446299

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
26
pubmed:dateCreated
2009-6-3
pubmed:abstractText
Formose sugar and formaldehyde (HCHO) were analyzed using high-performance liquid chromatography (HPLC) utilizing a CarboPac PA1 column (Dionex) and pulsed amperometric detection. This HPLC system was unsuitable for the analysis of formose sugar and HCHO and thus reducing sugars and unconverted HCHO were determined by endowing them with charges through a derivatization method using 2,4-dinitrophenylhydrazine. The separation and detection of compounds were performed by three Chromolith RP-C18 columns (Merck) and diode array detection, at a wavelength of 360 nm ultraviolet light, respectively. Lower sugars (except HCHO) showed some instabilities when the derivatized samples were kept for the extended periods of time. For C(5) and consecutive higher sugars, a certain derivatization time was necessary. In the present case (formose reaction with partial HCHO conversion), approximately 18 h may be a reasonable compromise for the derivatization reaction. A derivatization agent to compound mole ratio of up to 100:1 was required to complete the derivatization of C(4) and higher sugars. However, the analysis of C(4) and consecutive higher sugars is problematic for example due to overlapping of peaks or branched-chain sugars.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1873-3778
pubmed:author
pubmed:issnType
Electronic
pubmed:day
26
pubmed:volume
1216
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5116-21
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Analysis of formose sugar and formaldehyde by high-performance liquid chromatography.
pubmed:affiliation
Department of Food Science & Technology, University of Natural Resources and Applied Life Sciences (BOKU), Muthgasse 18, A-1190 Vienna, Austria. zafar.iqbal@boku.ac.at
pubmed:publicationType
Journal Article, Evaluation Studies