Linked Life Data

19446011

Source:http://linkedlifedata.com/resource/pubmed/id/19446011

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2009-7-3
pubmed:abstractText
We report on cellular actions of the illicit recreational drug gamma-hydroxybutyrate (GHB) in the brain reward area nucleus accumbens. First, we compared the effects of GHB and the GABA(B) receptor agonist baclofen. Neither of them affected the membrane currents of medium spiny neurons in rat nucleus accumbens slices. GABAergic and glutamatergic synaptic potentials of medium spiny neurons, however, were reduced by baclofen but not GHB. These results indicate the lack of GHB as well as postsynaptic GABA(B) receptors, and the presence of GHB insensitive presynaptic GABA(B) receptors in medium spiny neurons. In astrocytes GHB induced intracellular Ca(2+) transients, preserved in slices from GABA(B) receptor type 1 subunit knockout mice. The effects of tetrodotoxin, zero added Ca(2+) with/without intracellular Ca(2+) store depletor cyclopiazonic acid or vacuolar H-ATPase inhibitor bafilomycin A1 indicate that GHB-evoked Ca(2+) transients depend on external Ca(2+) and intracellular Ca(2+) stores, but not on vesicular transmitter release. GHB-induced astrocytic Ca(2+) transients were not affected by the GHB receptor-specific antagonist NCS-382, suggesting the presence of a novel NCS-382-insensitive target for GHB in astrocytes. The activation of astrocytes by GHB implies their involvement in physiological actions of GHB. Our findings disclose a novel profile of GHB action in the nucleus accumbens. Here, unlike in other brain areas, GHB does not act on GABA(B) receptors, but activates an NCS-382 insensitive GHB-specific target in a subpopulation of astrocytes. The lack of either post- or presynaptic effects on medium spiny neurons in the nucleus accumbens distinguishes GHB from many drugs and natural rewards with addictive properties and might explain why GHB has only a weak reinforcing capacity.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1873-7544
pubmed:author
pubmed:issnType
Electronic
pubmed:day
18
pubmed:volume
162
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
268-81
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:19446011-Animals, pubmed-meshheading:19446011-Astrocytes, pubmed-meshheading:19446011-Baclofen, pubmed-meshheading:19446011-Calcium, pubmed-meshheading:19446011-Dendritic Spines, pubmed-meshheading:19446011-Excitatory Postsynaptic Potentials, pubmed-meshheading:19446011-GABA-B Receptor Agonists, pubmed-meshheading:19446011-Inhibitory Postsynaptic Potentials, pubmed-meshheading:19446011-Male, pubmed-meshheading:19446011-Mice, pubmed-meshheading:19446011-Mice, Inbred BALB C, pubmed-meshheading:19446011-Neurons, pubmed-meshheading:19446011-Nucleus Accumbens, pubmed-meshheading:19446011-Rats, pubmed-meshheading:19446011-Rats, Wistar, pubmed-meshheading:19446011-Receptors, Cell Surface, pubmed-meshheading:19446011-Receptors, GABA-B, pubmed-meshheading:19446011-Sodium Oxybate
pubmed:year
2009
pubmed:articleTitle
gamma-Hydroxybutyrate (GHB) induces GABA(B) receptor independent intracellular Ca2+ transients in astrocytes, but has no effect on GHB or GABA(B) receptors of medium spiny neurons in the nucleus accumbens.
pubmed:affiliation
Department of Neurochemistry, Institute of Biomolecular Chemistry, Chemical Research Center, Hungarian Academy of Sciences, Pusztaszeri út 59-67.1025, Budapest, Hungary.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't