Source:http://linkedlifedata.com/resource/pubmed/id/19445974
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
7
|
pubmed:dateCreated |
2009-6-22
|
pubmed:abstractText |
Although coupled to G(i/o) proteins, cannabinoid CB(1) receptors can also activate intracellular Ca(2+) ([Ca(2+)](i)) accumulation through not fully understood mechanisms. We report that in, human neuroblastoma SH-SY5Y cells, CB(1) activation with the specific agonist arachidonoylchloroethanolamide (ACEA), weakly elevates [Ca(2+)](i) and that this effect, when using low (1-100 nM) concentrations of ACEA, is enhanced by the previous activation of G(q/11)-coupled M(3) muscarinic receptors with carbachol, dose-dependently and up to approximately 8-fold. A similar behaviour was also observed with carbachol and the G(i/o)-coupled delta-opioid receptor. Furthermore, stimulation of CB(1) receptors produced a concentration-dependent leftward shift of the elevation of [Ca(2+)](i) by delta-opioid receptors. These stimulatory effects were variedly attenuated by selective antagonists of each receptor, pertussis toxin, inhibitors of phospholipase C (U73122 and D609), and, when assessed in the presence of extracellular Ca(2+), by the block of voltage-activated calcium channels. Cholera toxin only slightly inhibited the G(q/11)-G(i/o)-mediated cross-talk, but induced a stronger inhibition of the G(i/o)-G(i/o)-mediated interaction. These findings suggest that activation of M(3) muscarinic receptors might produce a qualitative alteration of the signaling associated with G(i/o)-coupled receptors, and that sequential activation of CB(1) and delta-opioid receptors, both coupled to G(i/o), produces instead synergistic effects on [Ca(2+)](i) elevation.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Protein alpha...,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Protein alpha...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Cannabinoid, CB1,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Muscarinic M3,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Muscarinic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Opioid, delta
|
pubmed:status |
MEDLINE
|
pubmed:month |
Jul
|
pubmed:issn |
0006-3002
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
1793
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
1289-303
|
pubmed:dateRevised |
2009-11-19
|
pubmed:meshHeading |
pubmed-meshheading:19445974-Blotting, Western,
pubmed-meshheading:19445974-Calcium,
pubmed-meshheading:19445974-Fluorescent Antibody Technique,
pubmed-meshheading:19445974-GTP-Binding Protein alpha Subunits, Gi-Go,
pubmed-meshheading:19445974-GTP-Binding Protein alpha Subunits, Gq-G11,
pubmed-meshheading:19445974-Humans,
pubmed-meshheading:19445974-Neuroblastoma,
pubmed-meshheading:19445974-Receptor, Cannabinoid, CB1,
pubmed-meshheading:19445974-Receptor, Muscarinic M3,
pubmed-meshheading:19445974-Receptors, Muscarinic,
pubmed-meshheading:19445974-Receptors, Opioid, delta,
pubmed-meshheading:19445974-Tumor Cells, Cultured
|
pubmed:year |
2009
|
pubmed:articleTitle |
Cannabinoid CB1 receptor elevation of intracellular calcium in neuroblastoma SH-SY5Y cells: interactions with muscarinic and delta-opioid receptors.
|
pubmed:affiliation |
Institute of Biomolecular Chemistry, National Research Council, Via Campi Flegrei 34, Comprensorio Olivetti, Pozzuoli, Naples, Italy.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|