Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
21
pubmed:dateCreated
2009-5-27
pubmed:abstractText
Mesenteric fat is known to undergo inflammatory changes after 2,4,6,-trinitrobenzensulphonic acid (TNBS)-induced colitis. Neurotensin (NT) and neurotensin receptor 1 (NTR1) have been shown to play a major role in the pathogenesis of intestinal inflammation. This led us to explore whether NT and NTR1 are expressed in the mesenteric fat depots during TNBS-induced colitis and whether NT participates in the increased interleukin (IL)-6 secretion in this inflammatory response. TNBS-induced inflammation in the colon increases NT and NTR1 expression in mesenteric adipose tissues, including mesenteric preadipocytes. Compared with wild-type mice, NT knockout (KO) mice have reduced TNBS-induced colitis accompanied by diminished inflammatory responses in mesenteric adipose tissue. Specifically, IL-6 and p65 phosphorylation levels in mesenteric fat of NT KO mice are also reduced compared with wild-type mice. Mouse 3T3-L1 preadipocytes express NTR1 and its expression is increased after stimulation of preadipocytes with proinflammatory cytokines. NT stimulation of 3T3-L1 preadipocytes overexpressing NTR1 causes PKCdelta phosphorylation and IL-6 secretion in a time- and dose-dependent fashion. Moreover, NT-mediated IL-6 expression is nuclear factor-kappaB and PKCdelta dependent. We also found that supernatants from NT-exposed 3T3-L1-NTR1 preadipocytes and mesenteric fat obtained from wild-type mice 2 days after TNBS administration stimulate an IL-6-dependent macrophage migration measured by a macrophage migration assay, whereas this response is reduced when mesenteric fat from NT KO mice is used. These results demonstrate an important role for NT in acute colitis and adipose tissue inflammation associated with experimental colitis that involves direct NT proinflammatory responses in preadipocytes.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-10079105, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-10381912, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-10723677, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-10930370, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-11427716, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-11574537, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-11606501, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-12010776, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-12239100, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-12495721, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-12584113, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-12788800, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-14679176, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-14679177, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-15181022, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-15507533, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-15764810, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-15888774, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-15987829, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-16265231, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-16464856, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-16549770, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-16563502, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-16872719, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-17075601, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-17077645, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-17192460, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-17210639, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-17343284, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-17476678, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-17878371, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-18765478, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-9075932, http://linkedlifedata.com/resource/pubmed/commentcorrection/19443690-993203
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1091-6490
pubmed:author
pubmed:issnType
Electronic
pubmed:day
26
pubmed:volume
106
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8766-71
pubmed:dateRevised
2010-9-27
pubmed:meshHeading
pubmed-meshheading:19443690-3T3-L1 Cells, pubmed-meshheading:19443690-Adipocytes, pubmed-meshheading:19443690-Adipose Tissue, pubmed-meshheading:19443690-Animals, pubmed-meshheading:19443690-Cell Differentiation, pubmed-meshheading:19443690-Cell Movement, pubmed-meshheading:19443690-Colitis, pubmed-meshheading:19443690-Interleukin-6, pubmed-meshheading:19443690-Macrophages, pubmed-meshheading:19443690-Mice, pubmed-meshheading:19443690-Mice, Knockout, pubmed-meshheading:19443690-NF-kappa B, pubmed-meshheading:19443690-Neurotensin, pubmed-meshheading:19443690-Phosphorylation, pubmed-meshheading:19443690-Protein Kinase C-delta, pubmed-meshheading:19443690-Protein Subunits, pubmed-meshheading:19443690-Receptors, Neurotensin, pubmed-meshheading:19443690-Signal Transduction, pubmed-meshheading:19443690-Transcription, Genetic, pubmed-meshheading:19443690-Trinitrobenzenesulfonic Acid
pubmed:year
2009
pubmed:articleTitle
Neurotensin induces IL-6 secretion in mouse preadipocytes and adipose tissues during 2,4,6,-trinitrobenzensulphonic acid-induced colitis.
pubmed:affiliation
Division of Gastroenterology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
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