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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1991-12-13
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pubmed:databankReference |
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pubmed:abstractText |
We cloned a 1.2-kilobase cDNA (C17-16) from a transformed FRTL thyroid cell library. Northern blot analysis revealed that the size of the corresponding mRNA was 2.0 kilobases. C17-16 mRNA was found in all tissues investigated, but interestingly, its expression was 5- to 10-fold higher in the thyroid glands than in other tissues. Addition of TSH to FRTL cells showed a time- and dose-dependent increase in the steady state level of C17-16 mRNA, and the effect was mimicked by (Bu)2cAMP. An in vitro nuclear run-off assay demonstrated that the stimulatory effect was due to an increase in the transcription rate of the C17-16 gene. TSH had no effect on the half-life of the C17-16 mRNA. Transcriptional induction of the C17-16 gene was inhibited when the cells were treated with cycloheximide. Nucleotide sequencing revealed that C17-16 was identical to the cDNA of rat glucose-regulated protein (GRP78), a member of the heat shock protein family. These results suggest that the expression of GRP78 in thyroid cells is regulated by TSH via cAMP, for which cycloheximide-sensitive protein synthesis might be required, and that more GRP78 might be needed to assist in the synthesis and transport of glycoprotein molecules in TSH-stimulated cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0888-8809
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
905-10
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:1944297-Animals,
pubmed-meshheading:1944297-Base Sequence,
pubmed-meshheading:1944297-Blotting, Northern,
pubmed-meshheading:1944297-Carrier Proteins,
pubmed-meshheading:1944297-Cell Line,
pubmed-meshheading:1944297-Cloning, Molecular,
pubmed-meshheading:1944297-DNA,
pubmed-meshheading:1944297-Epithelium,
pubmed-meshheading:1944297-Gene Expression Regulation,
pubmed-meshheading:1944297-Half-Life,
pubmed-meshheading:1944297-Heat-Shock Proteins,
pubmed-meshheading:1944297-Immunoglobulin Heavy Chains,
pubmed-meshheading:1944297-Kinetics,
pubmed-meshheading:1944297-Molecular Chaperones,
pubmed-meshheading:1944297-Molecular Sequence Data,
pubmed-meshheading:1944297-RNA, Messenger,
pubmed-meshheading:1944297-Rats,
pubmed-meshheading:1944297-Thyroid Gland,
pubmed-meshheading:1944297-Thyrotropin,
pubmed-meshheading:1944297-Tissue Distribution,
pubmed-meshheading:1944297-Transcription, Genetic
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pubmed:year |
1991
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pubmed:articleTitle |
Thyrotropin stimulates glucose-regulated protein (GRP78) gene expression in rat functional thyroid epithelial cells, FRTL.
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pubmed:affiliation |
Third Department of Internal Medicine, University of Yamanashi Medical School, Japan.
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pubmed:publicationType |
Journal Article
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