Linked Life Data

19442606

Source:http://linkedlifedata.com/resource/pubmed/id/19442606

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2009-5-18
pubmed:abstractText
Injured growth plate cartilage is often repaired by bony tissue resulting in impaired bone growth in children. Using a rat injury model, our previous studies show that following the injury-induced initial inflammatory response, an influx of mesenchymal-like cells occurs within the growth plate injury site prior to formation of bony tissue. As platelet-derived growth factor (PDGF-BB) is a potent chemotactic factor of mesenchymal cells during skeletal tissue repair, we examined its role during the early fibrogenic response and the subsequent bony repair of injured growth plate. Following growth plate injury, rats received daily injection of the PDGF receptor (PDGFR) inhibitor, Imatinib, for 7 days. Immunohistochemical analysis of injured growth plate at day 1 showed the presence of PDGF-BB expression in some inflammatory cells, while at day 4 PDGFR was expressed by a proportion of the infiltrating mesenchymal cells at the injury site. By day 4, PDGFR inhibition reduced mesenchymal infiltrate (P<0.05); by day 14, Imatinib-treated rats exhibited less bony trabeculae and cartilaginous repair tissues, fewer osteoclasts and less bone marrow (BM) at the injury site, compared to vehicle controls (P<0.01). In vitro "scratch" migration assays with rat BM mesenchymal cells revealed that recombinant PDGF-BB increased cell migration into the "wound" (P<0.05), while Imatinib inhibited this chemotactic response. Quantitative RT-PCR analysis showed that Imatinib treatment decreased expression of the cartilage and bone related genes, Col2a1 and osteocalcin, respectively. These results suggest that PDGF-BB contributes to growth plate injury repair by promoting mesenchymal progenitor cell infiltration, the chondrogenic and osteogenic responses, and remodelling of the repair tissues.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1873-2763
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
878-85
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:19442606-Animals, pubmed-meshheading:19442606-Bone Marrow Cells, pubmed-meshheading:19442606-Cell Movement, pubmed-meshheading:19442606-Cell Proliferation, pubmed-meshheading:19442606-Collagen Type II, pubmed-meshheading:19442606-Gene Expression, pubmed-meshheading:19442606-Growth Plate, pubmed-meshheading:19442606-Immunohistochemistry, pubmed-meshheading:19442606-Male, pubmed-meshheading:19442606-Mesenchymal Stem Cells, pubmed-meshheading:19442606-Osteocalcin, pubmed-meshheading:19442606-Osteoclasts, pubmed-meshheading:19442606-Piperazines, pubmed-meshheading:19442606-Platelet-Derived Growth Factor, pubmed-meshheading:19442606-Pyrimidines, pubmed-meshheading:19442606-Rats, pubmed-meshheading:19442606-Rats, Sprague-Dawley, pubmed-meshheading:19442606-Receptor, Platelet-Derived Growth Factor beta, pubmed-meshheading:19442606-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:19442606-Stromal Cells
pubmed:year
2009
pubmed:articleTitle
Potential roles of growth factor PDGF-BB in the bony repair of injured growth plate.
pubmed:affiliation
Department of Orthopaedic Surgery, Women's and Children's Hospital, North Adelaide, Australia. rosa.chung@adelaide.edu.au
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't