Source:http://linkedlifedata.com/resource/pubmed/id/19441334
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2009-5-15
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| pubmed:abstractText |
Coplanar microdomains consisting of two organosilane self-assembled monolayers (SAMs) terminated with chemically reactive functional groups (e.g., aldehyde-[CHO] and carboxy-[COOH] groups) were prepared on Si substrates covered with native oxide (SiO2/Si). A SAM of triethoxysilylundecanal (TESUD) molecules was prepared by chemical vapor deposition on the SiO2/Si surfaces. A few of these samples were exposed to 172 nm vacuum-ultraviolet (UV) radiation for 5-60 min at 10(5) Pa. Various experimental techniques including water-contact angle measurements, ellipsometry, attenuated total reflection Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and atomic force microscopy confirmed that CHO terminal groups of the SAM have been photochemically converted to COOH groups. Based on this result, site-selective photoirradiation of the TESUD-SAM with the same radiation source was performed using a photomask. Lateral force microscopy images revealed that well-ordered microstructures of 5 x 5 microm2 square-shaped features are formed on the substrate. The difference in chemical reactivity was characterized by fluorescence microscopy using specific bonding between biotin hydrazide and streptavidin-conjugated quantum dots (QDs). The fluorescence microscopy images revealed that biotin hydrazide immobilized site-selectively on the masked CHO-terminated regions and not on the vacuum-UV irradiated regions. However, the latter regions showed chemical reactivity to biotin hydrazide after activation treatments using N-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride, and well-defined QDs patterns can be obtained within these regions. This suggests that the surface CHO groups of the vacuum-UV irradiated regions were transformed to COOH groups, and the activation treatments lead to the formation of active N-hydroxysuccinimidyl esters, which are reactive toward amine groups of biotin hydrazide.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
1533-4880
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
9
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
455-60
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| pubmed:year |
2009
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| pubmed:articleTitle |
Fabrication of well-defined microdomains composed of aldehyde- and carboxy-terminated self-assembled monolayers.
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| pubmed:affiliation |
National Institute of Advanced Industrial Science and Technology 2266-98 Anagahora, Shimo-shidami, Moriyama-ku, Nagoya 463-8560, Japan.
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| pubmed:publicationType |
Journal Article
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