Source:http://linkedlifedata.com/resource/pubmed/id/19437447
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
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| pubmed:dateCreated |
2009-7-16
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| pubmed:abstractText |
Signal transducers and activators of transcription-3 (Stat3) plays a central role in interleukin-6 (IL-6)-mediated cell proliferation by inhibiting apoptosis in a variety of cell types. MicroRNA-21 (miRNA-21), a ubiquitous miRNA, acts as an anti-apoptotic factor that seems to be indirectly but strictly linked to Stat3. In order to determine whether the IL-6 induced Stat3 anti-apoptosis pathway is linked with miRNA-21, we first determined the effects of recombinant mouse IL-6 on Stat3 expression, mouse embryo viability, and the mRNA levels of apoptosis related genes and miRNA-21 during mouse embryo development in vitro. Addition of 10 or 100 ng/ml of recombinant IL-6 to the culture medium did not affect the developmental ability of 2-cell stage embryos into blastocysts. However, total cell number was significantly increased and apoptosis was reduced in blastocyst stage embryos cultured in the presence of 100 ng/ml of recombinant IL-6. Furthermore, addition of recombinant IL-6 to the culture medium significantly increased the copy numbers of anti-apoptotic miRNA-21, up-regulated Bcl2l1, and down-regulated casp3. Similarly, the injection of mature miRNA-21 into cells up-regulated Bcl2l1 and down-regulated casp3. These results suggest that the induction of the Stat3 anti-apoptotic pathway by IL-6 is linked to miRNA-21 expression, which possibly results in the regulation of cell apoptosis in early mouse embryo development.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Apoptosis Regulatory Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/MIRN21 microRNA, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/MicroRNAs,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/STAT3 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Stat3 protein, mouse
|
| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
|
| pubmed:issn |
1098-2795
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
76
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
854-62
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| pubmed:meshHeading |
pubmed-meshheading:19437447-Animals,
pubmed-meshheading:19437447-Apoptosis,
pubmed-meshheading:19437447-Apoptosis Regulatory Proteins,
pubmed-meshheading:19437447-Blastocyst,
pubmed-meshheading:19437447-Cell Membrane,
pubmed-meshheading:19437447-Cell Nucleus,
pubmed-meshheading:19437447-Data Interpretation, Statistical,
pubmed-meshheading:19437447-Female,
pubmed-meshheading:19437447-Gene Expression,
pubmed-meshheading:19437447-Interleukin-6,
pubmed-meshheading:19437447-Mice,
pubmed-meshheading:19437447-MicroRNAs,
pubmed-meshheading:19437447-Microscopy, Confocal,
pubmed-meshheading:19437447-Microscopy, Fluorescence,
pubmed-meshheading:19437447-Phosphorylation,
pubmed-meshheading:19437447-RNA, Messenger,
pubmed-meshheading:19437447-Recombinant Proteins,
pubmed-meshheading:19437447-STAT3 Transcription Factor,
pubmed-meshheading:19437447-Signal Transduction
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| pubmed:year |
2009
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| pubmed:articleTitle |
A link between the interleukin-6/Stat3 anti-apoptotic pathway and microRNA-21 in preimplantation mouse embryos.
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| pubmed:affiliation |
Department of Animal Sciences, Chungbuk National University, Gaesin-dong Cheongju, Chungbuk 361-763, South Korea.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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