19435662

Source:http://linkedlifedata.com/resource/pubmed/id/19435662

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0567416, umls-concept:C1148673, umls-concept:C1511790
pubmed:issue	14
pubmed:dateCreated	2009-7-13
pubmed:abstractText	Determining the sequence specifity of DNA binding molecules is a non-trivial task. Here we describe the development of a platform for assaying the sequence specificity of DNA ligands using label free detection on high density DNA microarrays. This is achieved by combining Cognate Site Identification (CSI) with Fluorescence Intercalation Displacement (FID) to create CSI-FID. We use the well-studied small molecule DNA ligand netropsin to develop this high throughput platform. Analysis of the DNA binding properties of protein- and small molecule-based libraries with CSI-FID will advance the development of genome-anchored molecules for therapeutic purposes.
pubmed:grant	http://linkedlifedata.com/resource/pubmed/grant/CA041986, http://linkedlifedata.com/resource/pubmed/grant/CA078045, http://linkedlifedata.com/resource/pubmed/grant/GM069420, http://linkedlifedata.com/resource/pubmed/grant/R01 CA041986-24, http://linkedlifedata.com/resource/pubmed/grant/R01 CA133508-01A1, http://linkedlifedata.com/resource/pubmed/grant/R01 GM069420-03
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pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/9107377
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/DNA, http://linkedlifedata.com/resource/pubmed/chemical/Ethidium, http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes, http://linkedlifedata.com/resource/pubmed/chemical/Intercalating Agents, http://linkedlifedata.com/resource/pubmed/chemical/Ligands, http://linkedlifedata.com/resource/pubmed/chemical/Netropsin, http://linkedlifedata.com/resource/pubmed/chemical/Small Molecule Libraries
pubmed:status	MEDLINE
pubmed:month	Jul
pubmed:issn	1464-3405
pubmed:author	pubmed-author:AnsariAseem ZAZ, pubmed-author:BogerDale LDL, pubmed-author:HauschildKarl EKE, pubmed-author:StoverJames SJS
pubmed:issnType	Electronic
pubmed:day	15
pubmed:volume	19
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	3779-82
pubmed:dateRevised	2010-12-3
pubmed:meshHeading	pubmed-meshheading:19435662-Binding Sites, pubmed-meshheading:19435662-DNA, pubmed-meshheading:19435662-Ethidium, pubmed-meshheading:19435662-Fluorescent Dyes, pubmed-meshheading:19435662-Intercalating Agents, pubmed-meshheading:19435662-Ligands, pubmed-meshheading:19435662-Netropsin, pubmed-meshheading:19435662-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:19435662-Small Molecule Libraries
pubmed:year	2009
pubmed:articleTitle	CSI-FID: high throughput label-free detection of DNA binding molecules.
pubmed:affiliation	Genome Center of Wisconsin, Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
pubmed:publicationType	Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural