Linked Life Data

19432814

Source:http://linkedlifedata.com/resource/pubmed/id/19432814

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6A
pubmed:dateCreated
2010-7-23
pubmed:abstractText
Hepatoma-derived growth factor (HDGF) is a novel mitogenic growth factor that has been implicated in many different carcinomas. Its role in keloid biology has not yet been investigated. The present study is aimed at examining the role of HDGF in keloid pathogenesis. Immunohistochemical staining and Western blot analyses were used to examine in vivo localization and expression of HDGF in keloid and normal skin tissue. This was followed by the detection of HDGF expression in fibroblasts cultured in vitro and fibroblasts exposed to serum. To investigate the effect of epithelial-mesenchymal interactions, a two-chamber system was employed in which keratinocytes on membrane inserts were co-cultured with the fibroblasts. HDGF expression levels in all cell extracts and conditioned media were assayed through Western blot analysis. In another set of experiments, the effect of exogenous recombinant HDGF on keloid fibroblasts (KF) and normal fibroblasts (NF) was examined. Cell proliferation was assessed by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and by quantifying proliferating cell nuclear antigen (PCNA) expression. Downstream targets of HDGF were identified by detecting their expression through Western blot analysis. Our results indicate that there was an increase in HDGF expression in the dermis of keloid compared with normal skin tissue. The application of serum and epithelial-mesenchymal interactions did not seem to have any effect on intracellular HDGF expression levels. However, co-culturing keloid keratinocytes with KFs resulted in increased HDGF secretion when compared with monoculture or normal controls. Furthermore, treatment with exogenous recombinant HDGF was found to increase the proliferation of KFs, activate the extracellular signal-regulated kinase (ERK) pathway and up-regulate the secretion of vascular endothelial growth factor (VEGF).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1582-4934
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1328-37
pubmed:meshHeading
pubmed-meshheading:19432814-Cell Proliferation, pubmed-meshheading:19432814-Coculture Techniques, pubmed-meshheading:19432814-Culture Media, Conditioned, pubmed-meshheading:19432814-Dermis, pubmed-meshheading:19432814-Enzyme Activation, pubmed-meshheading:19432814-Epithelium, pubmed-meshheading:19432814-Extracellular Signal-Regulated MAP Kinases, pubmed-meshheading:19432814-Fibroblasts, pubmed-meshheading:19432814-Humans, pubmed-meshheading:19432814-Intercellular Signaling Peptides and Proteins, pubmed-meshheading:19432814-Intracellular Space, pubmed-meshheading:19432814-Keloid, pubmed-meshheading:19432814-Mesoderm, pubmed-meshheading:19432814-Models, Biological, pubmed-meshheading:19432814-Protein Transport, pubmed-meshheading:19432814-Recombinant Proteins, pubmed-meshheading:19432814-Serum, pubmed-meshheading:19432814-Signal Transduction, pubmed-meshheading:19432814-Vascular Endothelial Growth Factor A
pubmed:year
2010
pubmed:articleTitle
Hepatoma-derived growth factor and its role in keloid pathogenesis.
pubmed:affiliation
Graduate Programme in Bioengineering, National University of Singapore, Singapore, Singapore.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't