Linked Life Data

19427851

Source:http://linkedlifedata.com/resource/pubmed/id/19427851

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
15
pubmed:dateCreated
2009-7-31
pubmed:abstractText
In vertebrates, adipose tissue stores energy in the form of fat. Fat storage is tightly controlled by and dynamically balanced with energy expenditure under physiological settings; the perturbation of fat in either excess (obese) or deficit (lipodystrophy) has devastating pathologic consequences in the fueling of homeostasis and organismal fitness. The process by which fat storage is coordinated through positive and negative feedback signals is still poorly understood. To address potential mechanisms underlying fat storage we study a Caenorhabditis elegans Krüppel-like transcription factor, Ce-klf-3 and demonstrate that klf-3 is a hitherto unrecognized key regulator of fat metabolism in C. elegans. The Ce-klf-3 is highly expressed during larval development and predominantly present in intestine: the site of fat digestion, absorption, storage, and utilization. We found a strong positive correlation between klf-3 expression and fat deposition in a worm's intestine. Significantly, a klf-3 (ok1975) loss-of-function mutation, characterized by the deletion of a 1658-bp sequence spanning the 3' end of exon 2 through to the 5' end of exon 3 of klf-3, enhanced fat deposition in the intestine and caused severe defects in worm reproduction. Although klf-3 mutants seemed very similar to wild type worms in appearance and life span, 70% of mutants became semi-sterile, each producing 40-50 viable progenies, and the remaining 30% were rendered completely sterile toward adulthood. Notably, both mutant types displayed extensive deposition of fat in the intestine. Our study also demonstrates that klf-3 is critical for maintaining normal fatty acid composition by regulating genes involved in a fatty acid desaturation pathway. Strikingly, klf-3 mutant animals with impaired fatty acid beta-oxidation pathway genes resulted in fat accumulation in the mutant worm. We present the first clear in vivo evidence supporting essential regulatory roles of KLF-3 in fat storage in C. elegans and shed light on the human equivalent in disease-gene association.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1090-2422
pubmed:author
pubmed:issnType
Electronic
pubmed:day
10
pubmed:volume
315
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2568-80
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:19427851-Adipose Tissue, pubmed-meshheading:19427851-Amino Acid Sequence, pubmed-meshheading:19427851-Animals, pubmed-meshheading:19427851-Animals, Genetically Modified, pubmed-meshheading:19427851-Caenorhabditis elegans, pubmed-meshheading:19427851-Caenorhabditis elegans Proteins, pubmed-meshheading:19427851-Fatty Acid Desaturases, pubmed-meshheading:19427851-Fatty Acids, pubmed-meshheading:19427851-Humans, pubmed-meshheading:19427851-Intestines, pubmed-meshheading:19427851-Kruppel-Like Transcription Factors, pubmed-meshheading:19427851-Molecular Sequence Data, pubmed-meshheading:19427851-Mutation, pubmed-meshheading:19427851-Phenotype, pubmed-meshheading:19427851-Recombinant Fusion Proteins, pubmed-meshheading:19427851-Sequence Alignment, pubmed-meshheading:19427851-Sequence Homology, Amino Acid
pubmed:year
2009
pubmed:articleTitle
Mutation in Caenorhabditis elegans Krüppel-like factor, KLF-3 results in fat accumulation and alters fatty acid composition.
pubmed:affiliation
Laboratories of Developmental Biology, New York Blood Center, New York, NY 10065, USA.
pubmed:publicationType
Journal Article