Source:http://linkedlifedata.com/resource/pubmed/id/19427182
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
2010-4-13
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| pubmed:abstractText |
Vitamin E (alpha-tocopherol) is an essential fat-soluble nutrient with antioxidant properties. alpha-Tocopherol transfer protein (alpha-TTP), the product of the gene responsible for familial isolated vitamin E deficiency, plays an important role in maintaining the plasma alpha-tocopherol level by mediating the secretion of alpha-tocopherol by the liver. However, the mechanisms underlying hepatic alpha-tocopherol secretion are not fully understood. This study was undertaken to elucidate the mechanism of alpha-tocopherol re-efflux from hepatocytes, the cells that have the most important role in regulating plasma-alpha-tocopherol concentrations. From in vitro experiments using [(3)H]alpha-tocopheryl acetate and McARH7777 cells that stably express alpha-tocopherol transfer protein (alpha-TTP), the following results were obtained. First, addition of apolipoprotein A-I (apoA-I), a direct acceptor of the ATP-binding cassette transporter A1 (ABCA1)-secreted lipids, increased alpha-tocopherol secretion in a dose-dependent manner. Second, probucol, an antiatherogenic compound reported to be an inactivator of ABCA1 reduced hepatic alpha-tocopherol secretion. Third, ABCA1-RNAi suppressed hepatic alpha-tocopherol secretion. In a mouse in vivo experiment, addition of 1% probucol to the diet decreased plasma alpha-tocopherol concentrations. These results strongly suggest that ABCA1 is substantially involved in hepatic alpha-tocopherol secretion.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/APOA1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/ATP binding cassette transporter 1,
http://linkedlifedata.com/resource/pubmed/chemical/ATP-Binding Cassette Transporters,
http://linkedlifedata.com/resource/pubmed/chemical/Anticholesteremic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Apolipoprotein A-I,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cholesterol,
http://linkedlifedata.com/resource/pubmed/chemical/Probucol,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/alpha-Tocopherol,
http://linkedlifedata.com/resource/pubmed/chemical/alpha-tocopherol transfer protein
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| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
1873-4847
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2010 Elsevier Inc. All rights reserved.
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| pubmed:issnType |
Electronic
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| pubmed:volume |
21
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
451-6
|
| pubmed:dateRevised |
2010-10-11
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| pubmed:meshHeading |
pubmed-meshheading:19427182-ATP-Binding Cassette Transporters,
pubmed-meshheading:19427182-Animals,
pubmed-meshheading:19427182-Anticholesteremic Agents,
pubmed-meshheading:19427182-Apolipoprotein A-I,
pubmed-meshheading:19427182-Carrier Proteins,
pubmed-meshheading:19427182-Cell Line, Tumor,
pubmed-meshheading:19427182-Cholesterol,
pubmed-meshheading:19427182-Dose-Response Relationship, Drug,
pubmed-meshheading:19427182-Hepatocytes,
pubmed-meshheading:19427182-Male,
pubmed-meshheading:19427182-Mice,
pubmed-meshheading:19427182-Mice, Inbred C57BL,
pubmed-meshheading:19427182-Probucol,
pubmed-meshheading:19427182-RNA, Small Interfering,
pubmed-meshheading:19427182-RNA Interference,
pubmed-meshheading:19427182-Rats,
pubmed-meshheading:19427182-Time Factors,
pubmed-meshheading:19427182-alpha-Tocopherol
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| pubmed:year |
2010
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| pubmed:articleTitle |
ATP-binding cassette transporter A1 is involved in hepatic alpha-tocopherol secretion.
|
| pubmed:affiliation |
Department of Health Chemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan.
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| pubmed:publicationType |
Journal Article,
Comparative Study
|