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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2009-5-27
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pubmed:abstractText |
Extracellular nucleotides and their metabolites activate ionotropic P2X and metabotropic P2Y receptors on the surface of various types of cells. Here, we investigated the involvement of P2X and P2Y receptor-mediated signaling in TCR-dependent T cell activation. Murine T cells were activated by stimulation of TCR, and both CD25 expression and interleukin (IL)-2 production were observed in activated T cells. Ecto-nucleotidase apyrase and P2Y6 antagonist MRS2578 significantly blocked the increases of both CD25 expression and IL-2 production, and P2X7 antagonists A438079 and oxidized ATP inhibited IL-2 production rather than CD25 expression, suggesting the involvement of P2Y6 and P2X7 receptors in different processes of T cell activation. MRS2578 also blocked TCR-dependent elevation of cytosolic Ca2+ in T cells. The P2X7 and P2Y6 receptors were expressed in murine CD4 T cells. In conclusion, our results indicate that activation of P2Y6 and P2X7 receptors contributes to T cell activation via TCR.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/A-438079,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Isothiocyanates,
http://linkedlifedata.com/resource/pubmed/chemical/P2rx7 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Purinergic P2 Receptor Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Pyridines,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic P2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic P2X7,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Thiourea,
http://linkedlifedata.com/resource/pubmed/chemical/purinoceptor P2Y6
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1090-2104
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
10
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pubmed:volume |
384
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
512-8
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:19426712-Animals,
pubmed-meshheading:19426712-Calcium,
pubmed-meshheading:19426712-Isothiocyanates,
pubmed-meshheading:19426712-Lymphocyte Activation,
pubmed-meshheading:19426712-Male,
pubmed-meshheading:19426712-Mice,
pubmed-meshheading:19426712-Mice, Inbred BALB C,
pubmed-meshheading:19426712-Purinergic P2 Receptor Antagonists,
pubmed-meshheading:19426712-Pyridines,
pubmed-meshheading:19426712-Receptors, Antigen, T-Cell,
pubmed-meshheading:19426712-Receptors, Purinergic P2,
pubmed-meshheading:19426712-Receptors, Purinergic P2X7,
pubmed-meshheading:19426712-T-Lymphocytes,
pubmed-meshheading:19426712-Tetrazoles,
pubmed-meshheading:19426712-Thiourea
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pubmed:year |
2009
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pubmed:articleTitle |
Blockade of murine T cell activation by antagonists of P2Y6 and P2X7 receptors.
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pubmed:affiliation |
Faculty of Pharmaceutical Sciences, Tokyo University of Science (TUS), 2641 Yamazaki, Noda-shi, Chiba 278-8510, Japan. tsukim@rs.noda.tus.ac.jp
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pubmed:publicationType |
Journal Article
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