| pubmed-article:19426689 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19426689 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:19426689 | lifeskim:mentions | umls-concept:C0003209 | lld:lifeskim |
| pubmed-article:19426689 | lifeskim:mentions | umls-concept:C0003693 | lld:lifeskim |
| pubmed-article:19426689 | lifeskim:mentions | umls-concept:C0012472 | lld:lifeskim |
| pubmed-article:19426689 | lifeskim:mentions | umls-concept:C0205245 | lld:lifeskim |
| pubmed-article:19426689 | lifeskim:mentions | umls-concept:C1979928 | lld:lifeskim |
| pubmed-article:19426689 | lifeskim:mentions | umls-concept:C1515999 | lld:lifeskim |
| pubmed-article:19426689 | lifeskim:mentions | umls-concept:C1521840 | lld:lifeskim |
| pubmed-article:19426689 | lifeskim:mentions | umls-concept:C0526226 | lld:lifeskim |
| pubmed-article:19426689 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:19426689 | pubmed:dateCreated | 2009-5-11 | lld:pubmed |
| pubmed-article:19426689 | pubmed:abstractText | Garcinol (camboginol) from the fruit rind of Guttiferae species shows anti-carcinogenic and anti-inflammatory properties, but the underlying molecular mechanisms are unclear. Here we show that garcinol potently interferes with 5-lipoxygenase (EC 7.13.11.34) and microsomal prostaglandin (PG)E2 synthase (mPGES)-1 (EC 5.3.99.3), enzymes that play pivotal roles in inflammation and tumorigenesis. In cell-free assays, garcinol inhibited the activity of purified 5-lipoxygenase and blocked the mPGES-1-mediated conversion of PGH2 to PGE2 with IC50 values of 0.1 and 0.3 microM, respectively. Garcinol suppressed 5-lipoxygenase product formation also in intact human neutrophils and reduced PGE2 formation in interleukin-1beta-stimulated A549 human lung carcinoma cells as well as in human whole blood stimulated by lipopolysaccharide. Moreover, garcinol interfered with isolated cyclooxygenase (COX)-1 (EC 1.14.99.1, IC50 = 12 microM) and with the formation of COX-1-derived 12(S)-hydroxy-5-cis-8,10-trans-heptadecatrienoic acid and thromboxane B2 in human platelets. In contrast, neither Ca2+-ionophore (A23187)-induced arachidonic acid release in neutrophils nor COX-2 activity in A549 cells or whole blood, measured as formation of 6-keto PGF1alpha, or isolated human recombinant COX-2 were significantly affected by garcinol (< or = 30 microM). Together, the high potency of garcinol to selectively suppress PGE2 synthesis and 5-lipoxygenase product formation provides a molecular basis for the anti-inflammatory and anti-carcinogenic effects of garcinol and rationalizes its therapeutic use. | lld:pubmed |
| pubmed-article:19426689 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19426689 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19426689 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19426689 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19426689 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19426689 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19426689 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19426689 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19426689 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19426689 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19426689 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19426689 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19426689 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19426689 | pubmed:month | May | lld:pubmed |
| pubmed-article:19426689 | pubmed:issn | 1873-2968 | lld:pubmed |
| pubmed-article:19426689 | pubmed:author | pubmed-author:WerzOliverO | lld:pubmed |
| pubmed-article:19426689 | pubmed:author | pubmed-author:NorthoffHinna... | lld:pubmed |
| pubmed-article:19426689 | pubmed:author | pubmed-author:KoeberleAndre... | lld:pubmed |
| pubmed-article:19426689 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19426689 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:19426689 | pubmed:volume | 77 | lld:pubmed |
| pubmed-article:19426689 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19426689 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19426689 | pubmed:pagination | 1513-21 | lld:pubmed |
| pubmed-article:19426689 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
| pubmed-article:19426689 | pubmed:meshHeading | pubmed-meshheading:19426689... | lld:pubmed |
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| pubmed-article:19426689 | pubmed:meshHeading | pubmed-meshheading:19426689... | lld:pubmed |
| pubmed-article:19426689 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19426689 | pubmed:articleTitle | Identification of 5-lipoxygenase and microsomal prostaglandin E2 synthase-1 as functional targets of the anti-inflammatory and anti-carcinogenic garcinol. | lld:pubmed |
| pubmed-article:19426689 | pubmed:affiliation | Department for Pharmaceutical Analytics, Pharmaceutical Institute, University of Tuebingen, Auf der Morgenstelle 8, D-72076 Tuebingen, Germany. | lld:pubmed |
| pubmed-article:19426689 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19426689 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:19426689 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:19426689 | lld:pubmed |
