Linked Life Data

19424598

Source:http://linkedlifedata.com/resource/pubmed/id/19424598

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2009-5-8
pubmed:abstractText
One of the large Mafs, MafA protein, is a strong transactivator of insulin in pancreatic beta cells. Mafs are also known to play important roles in a variety of developmental and differentiation processes in many organs and tissues. Adipocytes are highly involved in insulin actions and glucose and lipid metabolism, and their proliferation and differentiation is regulated by coordination of several signal transduction and transcriptional factors, including members of the Maf family. To explore the role of MafA in adipocytes, we modified the MafA mRNA level in cultured adipocytes by the RNA interference technique and analyzed the resulting morphological changes and changes in expression of related genes. MafA siRNA was transfected into 3T3-L1 adipocytes. Expression of MafA was confirmed by real-time PCR and Western blotting. Expression of adipocytokines and transcriptional factors was also measured by real-time PCR. Cells were examined for morphological changes and lipid accumulation by microscopy. The MafA expression level in the MafA-siRNA-transfected pre-adipocytes was reduced by approximately 30% on day 0 pre-induction and by approximately 70% on day 3 post-induction, in comparison with stop-siRNA-transfected cells. Cell growth and lipid droplet accumulation were prevented by MafA mRNA suppression, and peroxisome proliferator-activated receptor (PPAR) gamma2 and CCAAT/enhancer-binding proteins (C/EBP)alpha, both of which are transcriptional factors essential for adipocyte differentiation, were down-regulated. Expression of the genes encoding the adipocytokines, adiponectin and adipsin was also suppressed. The results suggested a possible role of the transcriptional factor MafA in regulation of adipocyte function and differentiation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1107-3756
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
725-32
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Suppression of MafA mRNA with siRNA prevents adipose cell differentiation in 3T3-L1 cells.
pubmed:affiliation
Institute of Geriatrics, Tokyo Women's Medical University, 2-15-1 Shibuya, Shibuya-ku, Tokyo 150-0002, Japan. tsuchiya@kc.twmu.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't