Linked Life Data

19420923

Source:http://linkedlifedata.com/resource/pubmed/id/19420923

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2009-5-7
pubmed:abstractText
Giant cell tumor of bone (GCTB) is characterized cytogenetically by frequent telomeric associations (tas). To explore the mechanisms behind the formation of tas in GCTB and to investigate their karyotypic consequences, the frequencies of tas and clonal aberrations other than tas in 20 GCTBs were compared to telomere length and status, as assessed by quantitative PCR, fluorescence in situ hybridization (FISH), and expression levels of four genes involved in telomere maintenance. Based on the G-banding results, the tumors were divided into two groups, one with a high frequency of tas and one with a low frequency. Clonal aberrations were found to be restricted to the group with a high level of tas, and the same group showed a significantly larger reduction in telomere length in tumor cells compared to peripheral blood cells. Furthermore, 65 out of 66 tas analyzed by FISH were negative for telomeric sequences. The expression levels of TERT, TERF1, TERF2, and POT1 did not correlate with telomere length or the frequency of tas. Thus, the present findings provide strong support for the notion that decreased telomere length is a prerequisite for tas in GCTBs and that the clonal changes occurring in GCTBs are derived from tas.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1424-859X
pubmed:author
pubmed:copyrightInfo
Copyright 2009 S. Karger AG, Basel.
pubmed:issnType
Electronic
pubmed:volume
124
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
121-7
pubmed:meshHeading
pubmed-meshheading:19420923-Adolescent, pubmed-meshheading:19420923-Adult, pubmed-meshheading:19420923-Chromosome Aberrations, pubmed-meshheading:19420923-Chromosome Banding, pubmed-meshheading:19420923-Clone Cells, pubmed-meshheading:19420923-Female, pubmed-meshheading:19420923-Gene Expression Regulation, Neoplastic, pubmed-meshheading:19420923-Giant Cell Tumor of Bone, pubmed-meshheading:19420923-Humans, pubmed-meshheading:19420923-In Situ Hybridization, Fluorescence, pubmed-meshheading:19420923-Male, pubmed-meshheading:19420923-Middle Aged, pubmed-meshheading:19420923-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:19420923-Telomerase, pubmed-meshheading:19420923-Telomere, pubmed-meshheading:19420923-Telomere-Binding Proteins, pubmed-meshheading:19420923-Telomeric Repeat Binding Protein 2
pubmed:year
2009
pubmed:articleTitle
Telomeric associations correlate with telomere length reduction and clonal chromosome aberrations in giant cell tumor of bone.
pubmed:affiliation
Department of Clinical Genetics, University Hospital, Lund, Sweden. samuel.gebre-medhin@med.lu.se
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't