Linked Life Data

19420002

Source:http://linkedlifedata.com/resource/pubmed/id/19420002

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2009-7-2
pubmed:abstractText
The action of interleukin-6 (IL-6) impacts female reproduction. Although IL-6 was recently shown to inhibit cilia activity in human fallopian tubes in vitro, the molecular mechanisms underlying IL-6 signaling to tubal function remain elusive. Here, we investigate the cellular localization, regulation, and possible function of two IL-6 receptors (IL-6R alpha and gp130) in mouse and human fallopian tubes in vivo. We show that IL-6R alpha is restricted to the cilia of epithelial cells in both mouse and human fallopian tubes. Exogenous 17beta-estradiol (E(2)), but not progesterone (P(4)), causes a time-dependent decrease in IL-6R alpha expression, which is blocked by the estrogen receptor (ER) antagonist ICI-182,780. Exposure of different ER-selective agonists propyl-(1H)-pyrazole-1,3,5-triyl-trisphenol or 2,3-bis-(4-hydroxyphenyl)-propionitrile demonstrated an ER subtype-specific regulation of IL-6R alpha in mouse fallopian tubes. In contrast to IL-6R alpha, gp130 was detected in tubal epithelial cells in mice but not in humans. In humans, gp130 was found in the muscle cells and was decreased in the periovulatory and luteal phases during the reproductive cycles, indicating a species-specific expression and regulation of gp130 in the fallopian tube. Expression of tubal IL-6R alpha and gp130 in IL-6 knockout mice was found to be normal; however, E(2) treatment increased IL-6R alpha, but not gp130, in IL-6 knockout mice when compared with wild-type mice. Furthermore, expression levels of IL-6R alpha, but not gp130, decreased in parallel with estrogenic accelerated oocyte-cumulus complex (OCC) transport in mouse fallopian tubes. Our findings open the possibility that cilia-specific IL-6R alpha may play a role in the regulation of OCC transport and suggest an estrogen-regulatory pathway of IL-6R alpha in the fallopian tube.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1522-1563
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
297
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
C140-51
pubmed:meshHeading
pubmed-meshheading:19420002-Adult, pubmed-meshheading:19420002-Animals, pubmed-meshheading:19420002-Cilia, pubmed-meshheading:19420002-Cumulus Cells, pubmed-meshheading:19420002-Cytokine Receptor gp130, pubmed-meshheading:19420002-Dose-Response Relationship, Drug, pubmed-meshheading:19420002-Down-Regulation, pubmed-meshheading:19420002-Epithelial Cells, pubmed-meshheading:19420002-Estradiol, pubmed-meshheading:19420002-Estrogen Receptor Modulators, pubmed-meshheading:19420002-Estrogen Receptor alpha, pubmed-meshheading:19420002-Fallopian Tubes, pubmed-meshheading:19420002-Female, pubmed-meshheading:19420002-Humans, pubmed-meshheading:19420002-Injections, Intraperitoneal, pubmed-meshheading:19420002-Interleukin-6, pubmed-meshheading:19420002-Mice, pubmed-meshheading:19420002-Mice, Inbred C57BL, pubmed-meshheading:19420002-Mice, Knockout, pubmed-meshheading:19420002-Oocytes, pubmed-meshheading:19420002-Ovulation, pubmed-meshheading:19420002-Progesterone, pubmed-meshheading:19420002-Receptors, Interleukin-6, pubmed-meshheading:19420002-Signal Transduction, pubmed-meshheading:19420002-Species Specificity, pubmed-meshheading:19420002-Time Factors
pubmed:year
2009
pubmed:articleTitle
Downregulation of cilia-localized Il-6R alpha by 17beta-estradiol in mouse and human fallopian tubes.
pubmed:affiliation
Dept. of Physiology/Endocrinology, Institute of Neuroscience and Physiology, The Sahlgrenska Academy at Univ. of Gothenburg, Medicinareg 11, PO Box 434, Gothenburg 40530, Sweden. ruijin.shao@fysiologi.gu.se
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't