Linked Life Data

19419853

Source:http://linkedlifedata.com/resource/pubmed/id/19419853

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2009-5-19
pubmed:abstractText
An electrochemical stripping assay for ultrasensitive detection of target DNA was reported in this work. The protocol involved nanoporous gold (NPG) electrode modified with single-stranded DNA (ssDNA) and Au nanoparticles (Au-NPs) co-loaded with two kinds of ssDNA, one was reporter DNA which was complementary to the target DNA, the other modified with PbS nanoparticles (PbS-NPs) was signal DNA which was non-complemented, reducing the cross-reaction between the targets and reporter DNA on the same Au-NP. The amount of target DNA was determined by indirect determination of the amount of lead ions through differential pulse anodic stripping voltammetry (DPASV). This protocol could detect target DNA of as low as femtomolar and exhibited excellent selectivity against one-base mismatched DNA and non-complementary DNA. Under the optimum conditions, the anodic stripping peak current of lead demonstrated a good linear relationship with the target DNA concentration in the range of 9.0x10(-16) to 7.0x10(-14) M. A detection limit of 2.6x10(-16) M of target DNA was achieved.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1873-4235
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3113-9
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Ultrasensitive electrochemical detection of DNA based on PbS nanoparticle tags and nanoporous gold electrode.
pubmed:affiliation
Key Laboratory of Eco-chemical Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies