Linked Life Data

19419324

Source:http://linkedlifedata.com/resource/pubmed/id/19419324

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2009-11-5
pubmed:abstractText
Studies on primary osteocytes, which compose >90-95% of bone cells, embedded throughout the mineralized matrix, are a major challenge because of their difficult accessibility and the very rare models available in vitro. We engineered a 3D culture method of primary human osteoblast differentiation into osteocytes. These 3D-differentiated osteocytes were compared with 2D-cultured cells and with human microdissected cortical osteocytes obtained from bone cryosections. Human primary osteoblasts were seeded either within the interspace of calibrated biphasic calcium phosphate particles or on plastic culture dishes and cultured for 4 wk in the absence of differentiation factors. Osteocyte differentiation was assessed by histological and immunohistological analysis after paraffin embedding of culture after various times, as well as by quantitative RT-PCR analysis of a panel of osteoblast and osteocyte markers after nucleic acid extraction. Histological analysis showed, after only 1 wk, the presence of an osteoid matrix including many lacunae in which the cells were individually embedded, exhibiting characteristics of osteocyte-like cells. Real-time PCR expression of a set of bone-related genes confirmed their osteocyte phenotype. Comparison with plastic-cultured cells and mature osteocytes microdissected from human cortical bone allowed to assess their maturation stage as osteoid-osteocytes. This model of primary osteocyte differentiation is a new tool to gain insights into the biology of osteocytes. It should be a suitable method to study the osteoblast-osteocyte differentiation pathway, the osteocyte interaction with the other bone cells, and orchestration of bone remodeling transmitted by mechanical loading and shear stress. It should be used in important cancer research areas such as the cross-talk of osteocytes with tumor cells in bone metastasis, because it has been recently shown that gene expression in osteocytes is strongly affected by cancer cells of different origin. It could also be a very efficient tool for drug testing and bone tissue engineering applications.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1523-4681
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1927-35
pubmed:meshHeading
pubmed-meshheading:19419324-Biological Markers, pubmed-meshheading:19419324-Bone Morphogenetic Proteins, pubmed-meshheading:19419324-Bone and Bones, pubmed-meshheading:19419324-Calcium Phosphates, pubmed-meshheading:19419324-Cell Culture Techniques, pubmed-meshheading:19419324-Cell Differentiation, pubmed-meshheading:19419324-Cells, Cultured, pubmed-meshheading:19419324-Ceramics, pubmed-meshheading:19419324-Gene Expression Profiling, pubmed-meshheading:19419324-Gene Expression Regulation, pubmed-meshheading:19419324-Genetic Markers, pubmed-meshheading:19419324-Humans, pubmed-meshheading:19419324-Immunohistochemistry, pubmed-meshheading:19419324-Microdissection, pubmed-meshheading:19419324-Osteocytes, pubmed-meshheading:19419324-Parathyroid Hormone, pubmed-meshheading:19419324-Plastics, pubmed-meshheading:19419324-RANK Ligand
pubmed:year
2009
pubmed:articleTitle
Human primary osteocyte differentiation in a 3D culture system.
pubmed:affiliation
GEPITOS, Université Nice Sophia-Antipolis, CNRS, UFR de Médecine, Nice, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't