Linked Life Data

19414401

Source:http://linkedlifedata.com/resource/pubmed/id/19414401

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2009-5-5
pubmed:abstractText
A sesquiterpene lactone, eupalinin A, found in Eupatorium chinense L., exhibited a marked inhibitory effect on cell growth in HL60 cells. In a previous study, it was indicated that the intracellular ROS generation accompanying mitochondrial dysfunction was closely related to the autophagic cell death induced by the treatment with eupalinin A. By glutathione (GSH) pre-treatment, eupalinin A-induced cell growth inhibition was markedly reduced in a time- and dose-dependent manner. Eupalinin A reduced the intracellular GSH level in the early phase, but the GSH pre-treatment reduced this depression. Interestingly, the supplementation of GSH changed the cell death type from autophagic cell death to apoptotic cell death. Pre-treatment with GSH plus p38 MAP kinase inhibitor (SB203580) strongly diminished the eupalinin A-induced autophagic cell death compared with GSH pre-treatment, suggesting a negative regulation of p38 MAP kinase in this cell death type conversion. Taken together, intracellular ROS levels, including GSH, are crucial for the susceptibility to cell death and the determination of type of eupalinin A-induced cell death.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0250-7005
pubmed:author
pubmed:issnType
Print
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1449-57
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Intracellular glutathione regulates sesquiterpene lactone-induced conversion of autophagy to apoptosis in human leukemia HL60 cells.
pubmed:affiliation
Gifu International Institute of Biotechnology, 1-1 Naka-Fudogaoka, Kakamigahara, Gifu 504-0838, Japan. titoh@giib.or.jp
pubmed:publicationType
Journal Article